Evaluation of serum serotonin as a biomarker of intestinal inflammation in calves

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2025-05-05 DOI:10.1016/j.vetimm.2025.110947

Murat UZTİMÜR , Aysu Ece ŞENGÜL , Cennet Nur ÜNAL

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Abstract

The functions of serotonin have been demonstrated in detail in many different subjects such as aggression, depression and inflammation in human medicine. However, information about the functional effects of serotonin concentration in veterinary medicine is lacking. The aim of this study was to evaluate the use of serotonin as an inflammatory marker in calves with acute diarrhea. A total of 40 calves, 30 with acute diarrhea and 10 control animals were evaluated. In this study, etiological agents responsible for diarrhea in calves (rotavirus, coronavirus, E. coli, Giardia lamblia, and C. parvum) were identified using immunochromatographic rapid test kits. While serotonin analyses were performed with a high-performance liquid chromatogram, biochemical analyses were performed with an automatic chemistry device. Serotonin (P < 0.001), SAA (P < 0.001), WBC (P < 0.001) and HCT (P < 0.005) levels of calves with acute diarrhea are statistically significantly higher than the control group. In contrast, sodium (P < 0.011) levels of calves with acute diarrhea are significantly lower than the control group. In calves with acute diarrhea, serum serotonin concentration was determined as AUC 0.89; sensitivity 80 %; specificity 80 %, cut-off 135.63 µg/l and p < 0.001. In conclusion, in this study, serotonin concentration increased significantly in parallel with the increase in haptoglobulin and SAA concentration in calves with acute diarrhea, and thus the results obtained show that serotonin can be used as an inflammatory biomarker in calves.

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血清5 -羟色胺作为犊牛肠道炎症生物标志物的评价

在人类医学中，血清素的功能已经在许多不同的领域得到了详细的证明，比如攻击性、抑郁和炎症。然而，关于血清素浓度在兽药中的功能作用的信息是缺乏的。本研究的目的是评估血清素作为急性腹泻犊牛炎症标志物的作用。共对40头犊牛、30头急性腹泻和10头对照进行评价。在这项研究中，使用免疫层析快速检测试剂盒鉴定了犊牛腹泻的病原（轮状病毒、冠状病毒、大肠杆菌、贾第鞭毛虫和小孢子虫）。血清素分析采用高效液相色谱进行，生化分析采用自动化学装置进行。急性腹泻犊牛血清素（P <； 0.001）、SAA （P <； 0.001）、WBC （P <； 0.001）、HCT （P <； 0.005）水平均显著高于对照组。急性腹泻犊牛钠（P <； 0.011）水平显著低于对照组。犊牛急性腹泻时血清5 -羟色胺浓度AUC为0.89；灵敏度80 %;特异性80 %，截止值135.63µg/l， p值 <； 0.001。综上所述，在本研究中，犊牛急性腹泻中血清素浓度与haptoglobulin和SAA浓度同步显著升高，结果表明血清素可作为犊牛炎症生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.