Assessing the genetic profile of cytochrome P450 and glutathione S-transferases of patients diagnosed with acute myeloid leukemia

IF 1.8 Q3 HEMATOLOGY

Hematology, Transfusion and Cell Therapy Pub Date : 2025-04-01 DOI:10.1016/j.htct.2025.103759

Gilmar de Andrade França , Luciana Nardinelli , Ricardo Rodrigues Giorgi , Thiago Pagliarini , Otávio César Carvalho Guimarães Baiocchi , Elvira Deolinda Rodrigues Pereira Velloso , Wellington Fernandes da Silva Jr , Eduardo Magalhães Rego , Israel Bendit

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Abstract

Objective

This study aimed to determine the frequency of genetic alterations as deletions and duplications in cytochrome P450 (CYP450) and glutathione S-transferases (GST) genes, as well as to investigate whether there is a relationship between these alterations and neutrophilic hematologic recovery in adult patients diagnosed with acute myeloid leukemia.

Method

DNA samples from 70 patients diagnosed with acute myeloid leukemia were evaluated using the Multiplex Ligation-dependent Probe Amplification technique. The presence or absence of polymorphisms was compared regarding the time to neutrophilic recovery (neutrophil count ≥1.0 × 10⁹/L) using Kaplan-Meier curves, with the comparison between the curves being performed using the non-parametric log-rank test.

Results

The median age of the participants was 57 years, with a higher proportion of females (57.2%) and white individuals (61.4%)’. A total of 76 polymorphisms (CYP450 + GST) were identified, comprising 38 deletions and 38 duplications. Kaplan-Meier curves revealed that the neutrophilic recovery time was longer for the group with polymorphisms (p-value = 0.0056).

Conclusion

The study demonstrated that CYP450 and GST genes are polymorphic, and these polymorphisms may lead to longer neutrophilic recovery after induction treatment of acute myeloid leukemia remission.

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评估急性髓系白血病患者细胞色素P450和谷胱甘肽s转移酶的遗传谱

目的本研究旨在确定细胞色素P450 （CYP450）和谷胱甘肽s -转移酶（GST）基因缺失和重复的基因改变频率，并探讨这些改变是否与急性髓系白血病成年患者中性粒细胞血清学恢复有关。方法应用多重结扎依赖探针扩增技术对70例急性髓系白血病患者的dna样本进行检测。使用Kaplan-Meier曲线比较中性粒细胞恢复时间（中性粒细胞计数≥1.0 × 109/L）多态性的存在与否，曲线之间的比较使用非参数log-rank检验。结果参与者的中位年龄为57岁，女性（57.2%）和白人（61.4%）的比例较高。共鉴定出76个多态性（CYP450 + GST），包括38个缺失和38个重复。Kaplan-Meier曲线显示，多态性组中性粒细胞恢复时间较长（p值= 0.0056）。结论CYP450和GST基因存在多态性，这种多态性可能导致急性髓性白血病诱导缓解后中性粒细胞恢复时间延长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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