A rhodamine B-based fluorescent probe for Hg2+ and its analytical applications

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-05-03 DOI:10.1016/j.saa.2025.126331

Peijun Li , Liting Tang , Junjie Yang , Liqiang Yan

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Abstract

A rhodamine B-based off–on photochemical probe was developed for selective and sensitive mercury ion (Hg²⁺) detection. The probe features a simple synthesis route and demonstrates excellent dual-mode signaling through Hg²⁺-triggered colorimetric transition (colorless to pink) and fluorescence enhancement (580 nm). With low LODs of 4.4 μM (absorption spectroanalysis) 0.0023 μM (fluorescence analysis) and 2.6 μM (RGB analysis), and high anti-interference capability, this probe enables quantitative analysis and visual identification of Hg²⁺ in multiple samples, such as environmental water, plant tissues (onion roots), food samples (rice, mung bean sprouts) and natural Chinese herbs (pueraria and honeysuckle). To facilitate field applications, the probe was engineered into low-cost test strips, exhibiting distinct Hg²⁺-responsive color/fluorescence changes in complex media (beverages, soil leachates). A smartphone-assisted quantification platform was established by correlating chromaticity ratios (R/B) with Hg²⁺ concentrations. This integration eliminates reliance on bulky instrumentation, offering a portable solution for on-site Hg²⁺ monitoring.

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罗丹明b基Hg2+荧光探针及其分析应用

研制了一种基于罗丹明b的光化学探针，用于选择性、灵敏地检测汞离子（Hg2+）。该探针具有简单的合成路线，并通过Hg2+触发的比色转变（无色到粉红色）和荧光增强（580 nm）表现出出色的双模式信号传导。该探针具有低lod (4.4 μM（吸收光谱分析）、0.0023 μM（荧光分析）和2.6 μM （RGB分析），抗干扰能力强，可对环境水、植物组织（洋葱根）、食品样品（大米、绿豆芽）、天然中草药（葛根、金银花）等多种样品中的Hg2+进行定量分析和视觉识别。为了便于现场应用，探针被设计成低成本的试纸，在复杂介质（饮料，土壤渗滤液）中显示出明显的Hg2+响应颜色/荧光变化。通过将色度比（R/B）与Hg2+浓度相关联，建立智能手机辅助定量平台。这种集成消除了对笨重仪器的依赖，为现场Hg2+监测提供了便携式解决方案。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.