Target responsive-regulated CRISPR/Cas12a electrochemiluminescence sensing of salmonella typhimurium integrating ultrafine Pt NCs-anchored MXenes-boosted luminol/O2 system

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-05-05 DOI:10.1016/j.bios.2025.117558

Xiaobo Zhang , Xiuwen Wang , Longjiao Zhu , Jinyan Zhu , Qiuyue Zheng , Jingli Yuan , Wentao Xu , Jijuan Cao

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引用次数: 0

Abstract

Salmonella typhimurium (S. typhimurium), as common and highly pathogenic foodborne pathogen, poses a significant risk to public safety worldwide. The development of highly sensitive, rapid and on-site method for S. typhimurium analysis is urgently needed to prevent bacterial infections. Herein, we introduced a CRISPR/Cas12a-driven electrochemiluminescence (ECL) sensor based on luminol/O₂ binary systems for S. typhimurium detection, employing ultrafine Pt nanoclusters-anchored 2D delaminated-MXenes (Pt NCs/D-MXenes) as the co-reactant accelerator. The ultrathin D-MXenes support regulates the size and dispersibility of Pt NCs and facilitates the full exposure of active sites, and synergistic interactions between D-MXenes and Pt NCs improves electrocatalytic properties toward the reduction of O₂, which promotes the generation of ROS for boosting ECL emission. Using target responsive-regulated CRISPR/Cas12a system, the ECL sensor for S. typhimurium showed a broad concentration range from 10¹ to 10⁶ CFU/mL and limit of detection of 6 CFU/mL, with satisfactory recoveries in spiked-actual samples.

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靶向响应性调控的CRISPR/Cas12a电化学发光传感鼠伤寒沙门氏菌整合超细Pt ncs锚定mxenes -促进鲁米诺/O2系统

鼠伤寒沙门氏菌（S. typhimurium）是一种常见的高致病性食源性病原体，对全球公共安全构成重大威胁。开发高灵敏度、快速、现场的鼠伤寒沙门氏菌分析方法是预防细菌感染的迫切需要。本文采用超细Pt纳米簇锚定2D分层mxenes （Pt NCs/D-MXenes）作为助反应剂促进剂，设计了一种基于luminol/O2二元体系的CRISPR/ cas12a驱动的电化学发光（ECL）传感器，用于鼠伤寒沙门氏菌检测。超薄的D-MXenes支架调节了Pt NCs的大小和分散性，促进了活性位点的充分暴露，D-MXenes与Pt NCs之间的协同作用提高了电催化还原O2的性能，从而促进了ROS的产生，从而提高了ECL的排放。采用靶向响应调节的CRISPR/Cas12a系统，鼠伤寒沙门氏菌ECL传感器的检测浓度范围为101 ~ 106 CFU/mL，检出限为6 CFU/mL，在实际样品中具有令人满意的回收率。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.