Artificial Cells Capable of NO Generation with Light Controllable Membraneless Organelles for Melanoma Therapy

IF 27.4 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Advanced Materials Pub Date : 2025-05-06 DOI:10.1002/adma.202500242

Yingming Zhao, Shubin Li, Yanhao Liu, Chao Li, Jingjing Zhao, Yongshuo Ren, Wan Zhao, Xiangxiang Zhang, Xinyu Cui, Xuefeng Tang, Peipei Ren, Xiaojun Han

{"title":"Artificial Cells Capable of NO Generation with Light Controllable Membraneless Organelles for Melanoma Therapy","authors":"Yingming Zhao, Shubin Li, Yanhao Liu, Chao Li, Jingjing Zhao, Yongshuo Ren, Wan Zhao, Xiangxiang Zhang, Xinyu Cui, Xuefeng Tang, Peipei Ren, Xiaojun Han","doi":"10.1002/adma.202500242","DOIUrl":null,"url":null,"abstract":"Membraneless organelles (MLOs) formed by liquid–liquid phase separation exhibit diverse important biofunctions in cells. The construction of artificial cells containing MLOs with enhanced complexity and functions is still challenging. Here a light-responsive protein, Cry2olig-IDRs, is designed and purified to form MLOs upon light (488 nm) irradiation. They are capable of rapidly recruiting positively charged inducible nitric oxide synthase (iNOS<sup>+</sup>) from surroundings to regulate its activity for NO production. NO-artificial cells are constructed by encapsulating Cry2olig-IDRs and iNOS<sup>+</sup> into giant unilamellar vesicles, which are capable of rapid production of NO with high concentration due to the formation of MLOs upon light irradiation. NO-artificial cells are confirmed to possess the ability for melanoma tumor therapy in mice. These findings provide an efficient method for remotely regulating enzyme activity inside artificial cells, paving the path to build more sophisticated artificial cells for their biomedical applications.","PeriodicalId":114,"journal":{"name":"Advanced Materials","volume":"143 1","pages":""},"PeriodicalIF":27.4000,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Materials","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1002/adma.202500242","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Membraneless organelles (MLOs) formed by liquid–liquid phase separation exhibit diverse important biofunctions in cells. The construction of artificial cells containing MLOs with enhanced complexity and functions is still challenging. Here a light-responsive protein, Cry2olig-IDRs, is designed and purified to form MLOs upon light (488 nm) irradiation. They are capable of rapidly recruiting positively charged inducible nitric oxide synthase (iNOS⁺) from surroundings to regulate its activity for NO production. NO-artificial cells are constructed by encapsulating Cry2olig-IDRs and iNOS⁺ into giant unilamellar vesicles, which are capable of rapid production of NO with high concentration due to the formation of MLOs upon light irradiation. NO-artificial cells are confirmed to possess the ability for melanoma tumor therapy in mice. These findings provide an efficient method for remotely regulating enzyme activity inside artificial cells, paving the path to build more sophisticated artificial cells for their biomedical applications.

Abstract Image

查看原文本刊更多论文

光可控无膜细胞器产生NO的人造细胞用于黑色素瘤治疗

液-液相分离形成的无膜细胞器（MLOs）在细胞中具有多种重要的生物功能。构建具有增强复杂性和功能的MLOs人工细胞仍然具有挑战性。本文设计并纯化了一种光响应蛋白cry2oligo - idrs，使其在光（488nm）照射下形成MLOs。它们能够从周围环境中快速招募带正电荷的诱导型一氧化氮合酶（iNOS+），调节其产生NO的活性。NO-人工细胞是通过将cry2oligi - idrs和iNOS+包封在巨大的单层囊泡中构建而成的，这些囊泡在光照射下形成MLOs，能够快速产生高浓度的NO。no -人工细胞被证实具有治疗小鼠黑色素瘤的能力。这些发现提供了一种远程调节人工细胞内酶活性的有效方法，为构建更复杂的用于生物医学应用的人工细胞铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Advanced Materials 工程技术-材料科学：综合

CiteScore

43.00

自引率

4.10%

发文量

2182

审稿时长

2 months

期刊介绍： Advanced Materials, one of the world's most prestigious journals and the foundation of the Advanced portfolio, is the home of choice for best-in-class materials science for more than 30 years. Following this fast-growing and interdisciplinary field, we are considering and publishing the most important discoveries on any and all materials from materials scientists, chemists, physicists, engineers as well as health and life scientists and bringing you the latest results and trends in modern materials-related research every week.