Mycorrhizal fungi drive Cd and P allocation strategies for the co-planting system of hyperaccumulator S. nigrum and upland rice

IF 7.3 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution Pub Date : 2025-05-05 DOI:10.1016/j.envpol.2025.126382

Xu Yang , Qiuyu Chen , Zhuomin Jiang , Wenzhen Chen , Tuantuan Cui , Bohan Wu , Huashou Li , Rongliang Qiu

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Abstract

Arbuscular mycorrhizal fungi (AMF) enhance the remediation potential of hyperaccumulator-crop co-planting systems, yet the mechanisms governing cadmium (Cd) and phosphorus (P) allocation remain unclear. To investigate these strategies, pot experiments were conducted using Cd-contaminated soil (1.0 mg kg⁻¹ Cd) where the Cd hyperaccumulator Solanum nigrum (S. nigrum) was intercropped with upland rice under Funneliformis mosseae inoculation. Rhizospheric GRSP content, Cd/P allocation patterns, and microbial community structure were analyzed using in situ analysis using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), sequential chemical extraction, and 16S rRNA sequencing. Results showed that AMF increased total Cd accumulation in S. nigrum shoots by 25.37 % while reducing Cd uptake in rice shoots and roots by 45.18 % and 55.54 %, respectively. AMF also enhanced the P uptake rate of S. nigrum by 1.76 times compared to non-inoculated conditions, thereby increasing the total P accumulation in S. nigrum by 25.62 % under Cd stress. Conversely, AMF negatively impacted the P content and total P accumulation in neighboring rice. Rhizospheric GRSP content increased significantly, indicating AMF's role in reducing Cd availability for rice. In situ analysis of LA-ICP-MS confirmed lower Cd content in rice rhizosphere and root surfaces, with minimal effects on S. nigrum. Lower DTPA-Cd concentrations in the rhizosphere of intercropped rice further substantiated the mycorrhizal Cd-blocking effects of AMF. Furthermore, AMF inoculation was the principal factor influencing alterations in the bacterial community structure within the intercropping system, by increasing the abundance of phosphate-solubilizing bacteria (mainly Ramlibacter, Roseisolibacter, and Bacillus) in the rhizosphere. AMF reduced the relative abundance of metal-tolerant bacteria (primarily Flavisolibacter) in the S. nigrum rhizosphere while enhancing their presence in the rice rhizosphere. This work revealed the resource acquisition effect (especially P uptake) of AMF on S. nigrum, thereby promoting Cd uptake and its preferential strengthening of the Cd-defending effect of the intercropped rice.

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菌根真菌驱动超积累稻与旱稻共栽系统Cd和P的分配策略

丛枝菌根真菌（AMF）增强了超积累者-作物共栽系统的修复潜力，但镉（Cd）和磷(P)分配的机制尚不清楚。为了研究这些策略，在镉污染土壤（1.0 mg·kg-1 Cd）上进行盆栽试验，在接种苔藓漏斗虫的情况下，间作镉超富集植物黑茄（Solanum nigrum）和旱稻。采用激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）、序贯化学提取和16S rRNA测序技术，对根际GRSP含量、Cd/P分配规律和微生物群落结构进行原位分析。结果表明，AMF使黑穗槐新梢Cd累积量增加25.37%，使水稻新梢和根系Cd吸收量分别减少45.18%和55.54%。与未接种条件相比，AMF使黑曲霉对磷的吸收速率提高了1.76倍，使黑曲霉在Cd胁迫下的总磷积累量增加了25.62%。相反，AMF对邻近水稻磷含量和全磷积累呈负向影响。根际GRSP含量显著增加，表明AMF降低了水稻Cd有效性。LA-ICP-MS原位分析证实，水稻根际和根表面Cd含量较低，对黑穗病菌的影响最小。间作水稻根际DTPA-Cd浓度的降低进一步证实了AMF对菌根cd的阻断作用。此外，AMF接种是影响间作系统内细菌群落结构变化的主要因素，通过增加根际磷溶菌（主要是Ramlibacter、Roseisolibacter和芽孢杆菌）的丰度。AMF降低了黑穗病根际耐金属细菌（主要是黄酮类细菌）的相对丰度，而增加了它们在水稻根际的存在。本研究揭示了AMF对黑穗稻的资源获取效应（尤其是磷吸收），从而促进间作水稻对Cd的吸收，并优先加强间作水稻对Cd的防御作用。

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来源期刊

Environmental Pollution 环境科学-环境科学

CiteScore

16.00

自引率

6.70%

发文量

2082

审稿时长

2.9 months

期刊介绍： Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health. Subject areas include, but are not limited to: • Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies; • Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change; • Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects; • Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects; • Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest; • New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.