A ratiometric and rapid-response fluorescent nanoprobe based on modification of carbon quantum dots by naphthalimide for HClO bioimaging in living cells and mice

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-30 DOI:10.1016/j.saa.2025.126321

Kedian Xu, Ruiqing Liang, Ziqing Ye, Yunling Gao

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Abstract

A ratiometric and rapid-response fluorescent nanoprobe (NA-CQDs) for the detection of hypochlorous acid based on naphthalimide (NA) modified carbon quantum dots (CQDs) has been prepared. The nanoprobe NA-CQDs showed blue fluorescence with a unique yellow fluorescence in the presence of HClO, accompanying the emission wavelength change from 457 nm to 538 nm. The fluorescence detection limit for HClO was 99 nM with a linear range from 0.1 to 32 μM. The nanoprobe exhibited a rapid response to HClO within 20 s. The sensing mechanism might be attributed to the oxidation and cleavage of p-aminophenylether group on the nanoprobe NA-CQDs, leading to the FRET process from the CQDs to the NA due to enhanced ICT of the NA portion. A series of real applications were conducted including spiked real water samples, test paper strips, and fluorescence bioimaging in RAW 264.7 and HepG2 cells and nude mice, highlighting the potential application of NA-CQDs for future environmental sensing and biosensing in living things.

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基于萘酰亚胺修饰碳量子点的比例快速反应荧光纳米探针用于活细胞和小鼠HClO生物成像

以萘酰亚胺（NA）修饰的碳量子点（CQDs）为基础，制备了一种用于次氯酸检测的比例快速反应荧光纳米探针（NA-CQDs）。纳米探针NA-CQDs在HClO存在下呈现蓝色荧光和独特的黄色荧光，发射波长从457 nm变化到538 nm。HClO的荧光检测限为99 nM，线性范围为0.1 ~ 32 μM。纳米探针在20 s内对HClO反应迅速。这种传感机制可能是由于纳米探针NA-CQDs上对氨基苯醚基团的氧化和裂解，导致NA部分的ICT增强，导致CQDs到NA的FRET过程。在RAW 264.7和HepG2细胞及裸鼠中进行了加标水样、试纸条、荧光生物成像等一系列实际应用，突出了NA-CQDs在未来生物环境传感和生物传感方面的潜在应用。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.