Two Cytauxzoon species in Brazil? Ocelots (Leopardus pardalis) and pumas (Puma concolor) as reservoir for Cytauxzoon spp.: Molecular characterization in three Brazilian biomes

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-04-30 DOI:10.1016/j.vprsr.2025.101272

Joares A. May Jr , Vinícius Baggio-Souza , Laura Berger , Rafaela Mallmann-Bohn , Adeyldes Oliveira Reis , Aline Girotto-Soares , Raíssa Sepulvida , Jorge Salomão Junior , Leonardo Sartorello , Fabio Souza da Silva , Hugo Borghezan Mozerle , Marcos Adriano Tortato , Adriano Rodrigues Lagos , Felipe Viana Manzano , Fernando Vieira Machado , Jorge José Cherem , Renata Fagundes-Moreira , João F. Soares

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Abstract

Cytauxzoonosis. is tick-borne disease that can cause subclinical to fatal manifestations in felids worldwide. Our study aimed to molecularly characterize Cytauxzoon spp. in two felid species in three different Brazilian biomes. Blood samples were collected from 27 ocelots and 10 pumas during health monitoring in the Pantanal, Cerrado, and Amazon biomes. Conventional PCR and sequencing of the 18S rRNA and CytB genes were performed, followed by a Bayesian phylogenetic analysis. Of the 37 samples, 73.52 % of ocelots and 60 % of pumas tested positive for Cytauxzoon spp. Phylogenetic analysis confirmed the presence of Cytauxzoon brasiliensis and a strain geentically close to Cytauxzoon felis in pumas and ocelots. Different tick species, such as Amblyomma sculptum were found on infected animals, suggesting a potential vector role for the species. Results support that ocelots and pumas are possible natural reservoirs for Cytauxzoon spp. in different Brazilian biomes. Even so, we highlight that the ecological implications of habitat loss and anthropogenic pressures, may exacerbate the Cytauxzoon pathogenicity for wild felids. Further studies are needed to elucidate the epidemiology and impact of Cytauxzoon spp. on wildlife health.

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巴西的两种细胞株？豹猫（Leopardus pardalis）和美洲狮（Puma concolor）作为Cytauxzoon spp的宿主：三个巴西生物群系的分子特征

Cytauxzoonosis。是一种蜱传疾病，可在世界范围内的猫科动物中引起亚临床到致命的表现。本研究旨在对巴西3个不同生物群系的2个野外物种细胞株进行分子表征。在潘塔纳尔、塞拉多和亚马逊生物群落的健康监测期间，采集了27只豹猫和10只美洲狮的血液样本。对18S rRNA和CytB基因进行常规PCR和测序，然后进行贝叶斯系统发育分析。在37份样本中，73.52%的豹猫和60%的美洲狮检测到Cytauxzoon spp阳性，系统发育分析证实在美洲狮和豹猫中存在巴西繁殖细胞株和与猫科细胞繁殖细胞株基因接近的菌株。在受感染的动物身上发现了不同种类的蜱虫，如雕刻型浅腹虫，这表明该物种可能具有媒介作用。结果支持豹猫和美洲狮可能是巴西不同生物群系中Cytauxzoon spp.的天然宿主。尽管如此，我们强调生境丧失和人为压力的生态影响可能会加剧野生猫科动物的致病性。目前尚不清楚胞虫的流行病学及其对野生动物健康的影响。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).