Molecular characterization of Strongylida infecting goats and sheep in northeastern Brazil using cytochrome-c oxidase subunit I partial sequencing

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-05-02 DOI:10.1016/j.vprsr.2025.101274

Kerla J.L. Monteiro , Deiviane A. Calegar , Beatriz Coronato-Nunes , Jessica P. Santos , Elis Regina C. dos Reis , Polyanna A.A. Bacelar , Mariana Fonseca Rossi , Márcio N. Bóia , Fernando Araújo Monteiro , Filipe A. Carvalho-Costa , Lauren H. Jaeger

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引用次数: 0

Abstract

Strongylida parasitizes goats and sheep; increasing resistance to anthelmintics demands species characterization. This study aims to identify Strongylida of goats and sheep in northeastern Brazil, assessing genetic diversity. Seventeen specimens of Ovis aries and 25 of Capra hircus were studied; DNA was obtained from eggs shed in ruminants' feces. A ∼ 700 bp fragment of cytochrome c oxidase subunit 1 (cox1) was PCR-amplified and sequenced; a computational species delimitation analysis was performed; evolutionary significant units (ESUs) were defined using Poisson Tree Process (PTP) and multi-rate Poisson Tree Process (mPTP). Concerning Oesophagostomum, 2 sequences were Oesophagostomum columbianum (2 haplotypes) and 3 were from other species with no cox1 sequences described. This unidentified ESU (3 haplotypes; HD ± SD = 1.000 ± 0.272) was delimited by the PTP and mPTP algorithms, distant 74 mutational steps away from O. columbianum. Trichostrongylus colubriformis (n = 16; 15 haplotypes; haplotype diversity [HD] ± standard deviation [SD] = 0.991 ± 0.025) and Haemonchus contortus (n = 5; 5 haplotypes; HD ± SD = 1.000 ± 0.126) were also characterized. The helminth fauna of small ruminants is genetically diverse and can be monitored before slaughter. Multilocus genetic studies including nuclear targets should be performed to gain a clearer understanding of this diversity.

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利用细胞色素c氧化酶亚基I部分测序分析巴西东北部山羊和绵羊感染的圆线虫的分子特征

圆线虫寄生于山羊和绵羊；对驱虫药的抗性日益增强，需要对物种进行表征。本研究旨在鉴定巴西东北部山羊和绵羊的圆线虫，评估遗传多样性。对17只羊羊和25只山羊进行了研究；DNA是从反刍动物粪便中的卵中获得的。细胞色素c氧化酶亚基1 （cox1）的一个~ 700 bp片段被pcr扩增并测序；进行了计算物种划分分析；采用泊松树过程（PTP）和多速率泊松树过程（mPTP）定义进化显著单位（esu）。关于o食管stomum， 2个序列为o食管stomum columbianum（2个单倍型），3个序列来自其他物种，未发现cox1序列。这个身份不明的ESU(3个单倍型；HD±SD = 1.000±0.272)由PTP和mPTP算法划分，距离O. columbium 74个突变步长。色状毛线虫(n = 16；15个单;单倍型多样性[HD]±标准差[SD] = 0.991±0.025)和弯血蜱（Haemonchus tortortus） (n = 5；5个单;HD±SD = 1.000±0.126)。小型反刍动物的蠕虫动物群具有遗传多样性，可以在屠宰前进行监测。应该进行包括核靶点在内的多位点遗传研究，以更清楚地了解这种多样性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).