VvNAC33 functions as a key regulator of drought tolerance in grapevine by modulating reactive oxygen species production

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-04-29 DOI:10.1016/j.plaphy.2025.109971

Na Xu , Songlin Zhang , Xiaoming Zhou , Xiaoxuan Ma , Mohabaiti Ayiguzeli , Haixia Zhong , Fuchun Zhang , Chuan Zhang , Vivek Yadav , Xinyu Wu , Xindi Mei

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引用次数: 0

Abstract

Grapevine (Vitis vinifera L. and other Vitis spp.) is an important economic crop, but its yield and quality are severely affected by drought stress. NAC transcription factors, which play key roles in plant stress responses, have remained largely unexplored in grapevine drought tolerance. This study identified VvNAC33 as a drought-responsive candidate gene through transcriptomic analysis and demonstrated its role as a positive regulator of drought tolerance. VvNAC33 expression was significantly upregulated under drought stress. Subcellular localization and transcriptional activity analyses confirmed its nuclear localization and transcriptional activation potential. Overexpression of VvNAC33 in Arabidopsis thaliana and transient overexpression in grapevine enhanced drought tolerance, whereas virus-induced gene silencing increased drought sensitivity. This enhanced tolerance was associated with the activation of the antioxidant defense system, including superoxide dismutase, peroxidase, and catalase, which promoted reactive oxygen species scavenging and alleviated oxidative damage. The enhanced expression of VvCAT1, VvCu/ZnSOD, and VvPOD4 by VvNAC33 highlights its crucial role in regulating antioxidant gene expression under drought stress. These findings strongly support the role of VvNAC33 in drought tolerance and identify it as a potential molecular target for enhancing drought resistance in grapevine.

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VvNAC33是葡萄抗旱性的关键调控因子，通过调控活性氧的产生来调控葡萄的抗旱性

葡萄（Vitis vinifera L.和其他葡萄属植物）是重要的经济作物，但其产量和品质受到干旱胁迫的严重影响。NAC转录因子在植物抗旱性中起关键作用，但在葡萄抗旱性中仍未得到充分研究。本研究通过转录组学分析确定了VvNAC33是干旱响应的候选基因，并证明了其在干旱耐受性中的正调控作用。干旱胁迫下，VvNAC33表达显著上调。亚细胞定位和转录活性分析证实了其核定位和转录激活潜力。VvNAC33在拟南芥中的过表达和在葡萄中的短暂过表达增强了抗旱性，而病毒诱导的基因沉默增加了干旱敏感性。这种增强的耐受性与抗氧化防御系统的激活有关，包括超氧化物歧化酶、过氧化物酶和过氧化氢酶，它们促进了活性氧的清除，减轻了氧化损伤。VvNAC33增强VvCAT1、VvCu/ZnSOD和VvPOD4的表达，凸显了其在干旱胁迫下调控抗氧化基因表达的重要作用。这些发现有力地支持了VvNAC33在葡萄抗旱性中的作用，并将其确定为提高葡萄抗旱性的潜在分子靶点。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.