Gender-associated heterogeneity of F2RL3 methylation in peripheral blood highlights its associations with early-stage male lung cancer in the Chinese population

IF 2.4 3区医学 Q3 ONCOLOGY

Cancer Epidemiology Pub Date : 2025-05-05 DOI:10.1016/j.canep.2025.102834

Yunhui Qu , Xiuzhi Zhang , Man Liu , Feifei Liang , Longtao Ji , Jiao Su , Feifei Di , Jing Zhang , Zhi Wang , Jie Zhang , Zheng Zhang , Yutong Zhao , Fengqi Chen , Yutong Li , Wanjian Gu , Rongxi Yang , Liping Dai , Songyun Ouyang

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引用次数: 0

Abstract

Background

DNA methylation alteration in peripheral blood provides a promising approach for the diagnosis of cancers. We aimed to investigate the association between blood-based methylation of F2RL3 and lung adenocarcinoma (LUAD).

Methods

A total of 970 LUAD patients, 333 benign pulmonary nodes (BPNs), and 2413 normal controls (NCs) were included. The methylation levels of blood F2RL3 were measured by mass spectrometry. The gender-associated heterogeneity of blood F2RL3 methylation in LUAD was investigated. The blood F2RL3 methylation associations with male LUAD were assessed by logistic regression. The relations of blood F2RL3 hypomethylation to tumor size and lymph node involvement in male LUAD were also evaluated. Mann-Whitney and Kruskal-Wallis tests were conducted for the comparisons. Tissue F2RL3 methylation and expression in LUAD were investigated via UALCAN database analysis.

Results

Blood-based F2RL3 methylation presented significant difference between male and female LUAD patients. While, blood F2RL3 methylation in LUAD patients were significantly lower than in BPN cases and NCs in male population. Through logistic regression analysis, the independent associations of blood F2RL3 hypomethylation with male LUAD was identified. In addition, blood F2RL3 methylation was correlated with tumor size and lymph nodes involvement in male LUAD patients.

Conclusions

In conclusion, blood-based F2RL3 methylation might be a potential biomarker for male LUAD early detection.

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外周血中F2RL3甲基化的性别相关异质性突出了其与中国人群早期男性肺癌的相关性

外周血dna甲基化改变为癌症的诊断提供了一种有希望的方法。我们的目的是研究血液中F2RL3甲基化与肺腺癌（LUAD）之间的关系。方法970例LUAD患者，333例良性肺淋巴结（BPNs）， 2413例正常对照（nc）。采用质谱法测定血中F2RL3的甲基化水平。研究了LUAD患者血液F2RL3甲基化的性别相关异质性。通过logistic回归评估血液F2RL3甲基化与男性LUAD的关联。我们还评估了男性LUAD患者血液F2RL3低甲基化与肿瘤大小和淋巴结累及的关系。进行了Mann-Whitney和Kruskal-Wallis测试进行比较。通过UALCAN数据库分析研究LUAD组织中F2RL3的甲基化和表达。结果血液F2RL3甲基化在男女LUAD患者中存在显著差异。而LUAD患者血液中F2RL3甲基化水平明显低于男性BPN患者和NCs患者。通过logistic回归分析，确定了血液F2RL3低甲基化与男性LUAD的独立关联。此外，血液F2RL3甲基化与男性LUAD患者的肿瘤大小和淋巴结累及相关。结论血液F2RL3甲基化可能是男性LUAD早期检测的潜在生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Epidemiology 医学-肿瘤学

CiteScore

4.50

自引率

3.80%

发文量

200

审稿时长

39 days

期刊介绍： Cancer Epidemiology is dedicated to increasing understanding about cancer causes, prevention and control. The scope of the journal embraces all aspects of cancer epidemiology including: • Descriptive epidemiology • Studies of risk factors for disease initiation, development and prognosis • Screening and early detection • Prevention and control • Methodological issues The journal publishes original research articles (full length and short reports), systematic reviews and meta-analyses, editorials, commentaries and letters to the editor commenting on previously published research.