One-step synthesis of strongly stabilized yellow fluorescent carbon dots for rapid Golgi apparatus targeting and imaging

IF 4.3 3区材料科学 Q2 MATERIALS SCIENCE, COATINGS & FILMS

Diamond and Related Materials Pub Date : 2025-05-02 DOI:10.1016/j.diamond.2025.112392

Hao Li , Zihao Zhou , Chuyao Ni , Jianwu Xiao , Jun Cao , Jiaqi Pan , Chaorong Li , Yingying Zheng

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引用次数: 0

Abstract

Targeted monitoring of the Golgi apparatus (GA) morphology and structure as well as the Golgi apparatus stress response is both essential and challenging. Golgi-targeting phenylsulfonamide-rich carbon dots (PR-CDs) with excitation-dependent, lower cytotoxicity and favorable biocompatibility were synthesized by one-step solvothermal process. Phenylsulfonamide unit which had a strong binding affinity for the inducible enzyme (cyclooxygenase-2 (COX-2)) expressed and accumulated on the Golgi apparatus surface was introduced as a Golgi-targeting moiety. PR-CDs had abundant surface functional groups (amino group, phenylsulfonamide and sulfonic acid group) and excellent fluorescence stability in complex environments (PH, amino acid, ionic liquid, polarity and viscosity). Rapid and accurate imaging of the Golgi apparatus in living cells was achieved by PR-CDs within 15 min and yielded an average Pearson's coefficient of 0.92. Additionally, efficient tracking and imaging of the Golgi apparatus during apoptosis cell death were also accomplished. The successful preparation of PR-CDs provides an innovative subcellular targeting strategy and the prepared PR-CDs could serve as excellent carriers for targeted drug delivery in the future.

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用于高尔基体快速瞄准成像的强稳定黄色荧光碳点一步合成

有针对性地监测高尔基体（GA）的形态和结构以及高尔基体的应激反应是必不可少的，也是具有挑战性的。采用一步溶剂热法合成了高尔基靶富苯磺酰胺碳点（PR-CDs），该碳点具有激发依赖性、低细胞毒性和良好的生物相容性。引入对高尔基体表面表达和积累的诱导酶（环氧化酶-2 (COX-2)）具有较强结合亲和力的苯磺酰胺单元作为高尔基靶段。PR-CDs具有丰富的表面官能团（氨基、苯磺酰胺和磺酸基），在复杂环境（PH、氨基酸、离子液体、极性和粘度）下具有优异的荧光稳定性。pr - cd在15分钟内实现了活细胞中高尔基体的快速准确成像，平均Pearson系数为0.92。此外，还实现了高尔基体在细胞凋亡过程中的有效跟踪和成像。PR-CDs的成功制备提供了一种创新的亚细胞靶向策略，制备的PR-CDs可以作为未来靶向药物递送的优秀载体。

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来源期刊

Diamond and Related Materials 工程技术-材料科学：综合

CiteScore

6.00

自引率

14.60%

发文量

702

审稿时长

2.1 months

期刊介绍： DRM is a leading international journal that publishes new fundamental and applied research on all forms of diamond, the integration of diamond with other advanced materials and development of technologies exploiting diamond. The synthesis, characterization and processing of single crystal diamond, polycrystalline films, nanodiamond powders and heterostructures with other advanced materials are encouraged topics for technical and review articles. In addition to diamond, the journal publishes manuscripts on the synthesis, characterization and application of other related materials including diamond-like carbons, carbon nanotubes, graphene, and boron and carbon nitrides. Articles are sought on the chemical functionalization of diamond and related materials as well as their use in electrochemistry, energy storage and conversion, chemical and biological sensing, imaging, thermal management, photonic and quantum applications, electron emission and electronic devices. The International Conference on Diamond and Carbon Materials has evolved into the largest and most well attended forum in the field of diamond, providing a forum to showcase the latest results in the science and technology of diamond and other carbon materials such as carbon nanotubes, graphene, and diamond-like carbon. Run annually in association with Diamond and Related Materials the conference provides junior and established researchers the opportunity to exchange the latest results ranging from fundamental physical and chemical concepts to applied research focusing on the next generation carbon-based devices.