Flooding affects the synthesis of amino acids in purple rice grains

Abstract

Flooding disrupts plant metabolism and affecting amino acid synthesis. This study used non targeted metabolomics to investigate different flood levels (NF: control, MF: mild flooding, SF: severe flooding) on the synthesis and quantification of amino acids in purple rice. The findings indicated that as flooding intensity increased, the levels of most amino acids progressively declined. Compared to NF, SF exhibited a significant reduction in amino acid content, particularly with Glu, Asp, and Val decreasing by approximately 53 %, 55 %, and 56 %, respectively, confirming the inhibitory effect of flooding on amino acid synthesis. In the amino acid synthesis pathway, L-aspartic acid, L-phenylalanine, L-proline, and L-homoserine were found to be down regulated metabolites in MF and NF, while L-phenylalanine, Citric acid, L-aspartic acid, and L-homoserine were down regulated metabolites in SF and NF. The research results indicate that flooding has a significant inhibitory effect on the synthesis of amino acids in rice grains.