Generation of human induced pluripotent stem cell lines from two down syndrome patients, including a down syndrome/Alzheimer’s disease case (FLENIi002-A) and a beta-amyloid-resistant case (FLENIi003-A)

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-05-01 DOI:10.1016/j.scr.2025.103728

Giulia S. Clas , Mariela Marazita , Lucía Pertierra , Silvia Vazquez , Luciana Isaja , Soledad Rodríguez-Varela , Sofía Mucci , Belén Helou , Fernanda Tapajóz , Nahuel Magrath , Yanina Bérgamo , Ricardo Allegri , Gustavo E. Sevlever , Maria E. Scassa , Ezequiel I. Surace , Leonardo Romorini

引用次数: 0

Abstract

Two human induced pluripotent stem cell (hiPSC) lines, FLENIi002-A and FLENIi003-A, were generated from peripheral blood mononuclear cells (PBMCs) using the lentiviral-hSTEMCCA-loxP vector. FLENIi002-A was derived from a 52-year-old Down syndrome patient with Alzheimer’s disease and amyloid-beta brain accumulation. FLENIi003-A was derived from a cognitively unimpaired 51-year-old Down syndrome patient exhibiting no brain amyloid-beta deposition. Both lines retained the trisomy 21 genotype, and their pluripotency and differentiation potential were confirmed.

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从两名唐氏综合征患者（包括一名唐氏综合征/阿尔茨海默病病例（FLENIi002-A）和一名β -淀粉样蛋白耐药病例（FLENIi003-A））中生成人类诱导多能干细胞系

利用慢病毒- hstemcca - loxp载体，从外周血单个核细胞（PBMCs）中生成两种人诱导多能干细胞（hiPSC）系FLENIi002-A和FLENIi003-A。FLENIi002-A来源于一名52岁患有阿尔茨海默病和β淀粉样蛋白脑积聚的唐氏综合征患者。FLENIi003-A来源于一名认知功能未受损的51岁唐氏综合征患者，没有脑淀粉样蛋白沉积。两系均保留21三体基因型，证实了其多能性和分化潜力。

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.