Effect of fine molecular structure of debranched starch on its digestibility

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-04-30 DOI:10.1016/j.ijbiomac.2025.143703

Lu Yang , Ruqing Sun , Zihui Xu , Yang Qin , Liu Xiong , Rui Shi , Qingjie Sun

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Abstract

Debranched starch (DBS) has shown promise in the development of slow-digesting starchy foods due to its abundant short-chain glucans (SCGs). However, the effect of its fine molecular structure on digestibility remains unclear. In this study, DBS with varying contents of partially debranched amylopectin (PDA) and debranched SCGs were obtained by adjusting the enzyme content and hydrolysis time, followed by analysis of their multiscale structure and digestibility. The results showed that as the SCGs content increased from 54.16 % to 100 %, the recrystallization rate of DBS significantly increased from 0.19 to 0.62, accompanied by a morphological transition from a worm-like to a spherical structure. SAXS analysis confirmed that high SCGs content led to an ordered and dense DBS structure. Correlation analysis revealed stage-dependent effects of debranching degree and B2 + B3 chain ratio on DBS digestibility: B2 + B3 chains primarily enhanced resistant starch (RS) content when SCGs <85 %, whereas both factors synergistically increased both slowly digestible starch and RS content at SCGs >85 %. These findings suggest that modulating the chain length distribution of DBS is an effective strategy for tailoring its digestive characteristics.

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脱支淀粉精细分子结构对其消化率的影响

由于其丰富的短链葡聚糖（SCGs），脱支淀粉（DBS）在慢消化淀粉类食物的开发中显示出前景。然而，其精细的分子结构对消化率的影响尚不清楚。本研究通过调整酶的含量和水解时间，获得了不同含量的部分脱支支链淀粉（PDA）和脱支支链淀粉（SCGs），并对其进行了多尺度结构和消化率分析。结果表明，当SCGs含量从54.16%增加到100%时，DBS的再结晶率从0.19显著提高到0.62，并伴有从蠕虫状结构到球形结构的形态转变。SAXS分析证实，高SCGs含量导致DBS结构有序且致密。相关分析显示，脱分枝度和B2 + B3链比对DBS消化率有阶段性影响：当SCGs <； 85%时，B2 + B3链主要提高了抗性淀粉（RS）含量，而在SCGs >； 85%时，这两个因素协同提高了慢消化淀粉和RS含量。这些发现表明，调节DBS的链长分布是调整其消化特性的有效策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.