In vitro assessment of the effect of Porphyra haitanensis polysaccharides on the intestinal flora of allergic mice

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-05-05 DOI:10.1016/j.ijbiomac.2025.143950

Peilin Chen , YuShan Huang , Hongliang Zeng , Mingjing Zheng , Juanjuan Guo

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Abstract

This study systematically investigated the modulatory effects of Porphyra haitanensis polysaccharides (PHP) and its two P. haitanensis polysaccharide components (PHP40 and PHP80) on gut microbiota in ovalbumin (OVA)-sensitized mice via in vitro fermentation. PHP40 and PHP80 exhibited sulfate contents of 5.94 ± 0.05 % and 11.72 ± 0.03 %, respectively, with galactose and glucose as dominant monosaccharides. The different polysaccharide components had different effects on the aforementioned changes in the species composition and structure of the gut microbiota in allergic mice, with distinct dominant microbial profiles across groups. PHP could promote Bacillus and Enterococcus proliferation while inhibiting Staphylococcus. PHP40 and PHP80 could promote Enterococcus and Enterobacter growth but suppressed Staphylococcus and Bacillus. Functional prediction indicated PHP significantly improved galactose metabolism and primary/secondary bile acid biosynthesis, potentially alleviating allergic responses.

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海棠紫菜多糖对过敏性小鼠肠道菌群影响的体外研究

本研究通过体外发酵系统地研究了海檀卟多糖（PHP）及其两种海檀多糖成分（PHP40和PHP80）对卵清蛋白（OVA）致敏小鼠肠道菌群的调节作用。PHP40和PHP80的硫酸盐含量分别为5.94±0.05%和11.72±0.03%，以半乳糖和葡萄糖为优势单糖。不同的多糖成分对上述过敏小鼠肠道菌群的种类组成和结构变化有不同的影响，各组间优势菌群分布不同。PHP能促进芽孢杆菌和肠球菌的增殖，抑制葡萄球菌的增殖。PHP40和PHP80能促进肠球菌和肠杆菌的生长，抑制葡萄球菌和芽孢杆菌的生长。功能预测表明，PHP可显著改善半乳糖代谢和初级/次级胆汁酸生物合成，可能减轻过敏反应。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.