Validated HPLC-UV Detection Method for Cefiderocol Quantification in a Critical Patient Receiving Fetcroja and Treated With Extracorporeal Replacement Therapies

IF 1.8 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-05-04 DOI:10.1002/bmc.70104

Carlos Ezquer-Garin, Rafael Ferriols-Lisart, Manuel Alós-Almiñana, J. A. Carbonell, L. Hurtado, L. García-Vargas, G. Aguilar

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Abstract

A simple, rapid, and sensitive HPLC-UV method for cefiderocol quantification in plasma was developed to study the pharmacokinetic profile in a critical patient receiving Fetcroja and treated with a renal replacement therapy. After a quick deproteinization step by an effective precipitation, a chromatographic separation was performed with a C18 column and a mobile phase composed by acetonitrile and phosphate buffer at pH 3.50, delivered by gradient elution at a flow rate of 1.0 mL min⁻¹. The UV detector was set at 260 nm. Metronidazole was used as an internal standard. A total run time analysis of 15 min was obtained. The method was validated according to the standard guidelines and applied to study the pharmacokinetics of cefiderocol in a critical patient. Total run time analysis obtained was shorter than previously HPLC reported methods, being useful for therapeutic drug monitoring or pharmacokinetic profile research.

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经验证的高效液相色谱-紫外检测法在接受体外替代治疗的重症患者中定量头孢地罗

建立了一种简单、快速、灵敏的HPLC-UV定量血浆中头孢地罗的方法，以研究接受Fetcroja并接受肾脏替代治疗的危重患者的药代动力学特征。通过有效沉淀进行快速脱蛋白步骤后，用C18柱和流动相进行色谱分离，流动相由乙腈和磷酸盐缓冲液组成，pH为3.50，梯度洗脱，流速为1.0 mL min - 1。紫外检测器设置为260 nm。以甲硝唑为内标。总运行时间分析为15分钟。根据标准指南对方法进行了验证，并应用于头孢地罗在危重患者体内的药动学研究。获得的总运行时间分析比以前报道的高效液相色谱方法短，可用于治疗药物监测或药代动力学谱研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.