Identification of Aerobic Salivary Microorganisms in Patients With Oral Squamous Cell Carcinoma Using MALDI-TOF MS: Preliminary Findings From a Pilot Study

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Rapid Communications in Mass Spectrometry Pub Date : 2025-05-05 DOI:10.1002/rcm.10063

Milena da Silva Moreira, Giovanna Schmitz, Mariana de Sá Alves, Maria Anita Mendes, Mônica Ghislaine Oliveira Alves, Levy Anderson César Alves, Meriellen Dias, Celso Muller Bandeira, Gabrielle Luana Jimenez Teodoro Nepomuceno, Herculano da Silva Martinho, Janete Dias Almeida

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引用次数: 0

Abstract

Rationale

Oral squamous cell carcinoma (OSCC) is a multifactorial disease, and emerging evidence links the oral microbiome to its development. Rapid, noninvasive identification of salivary microorganisms may offer novel diagnostic and prognostic insights into oral cancer.

Methods

Salivary microbiota from OSCC patients and healthy individuals were analyzed using MALDI Biotyper. Saliva samples were cultured, and microbial identification was performed based on protein spectral profiles using an UltrafleXtreme MALDI-TOF mass spectrometer.

Results

Thirteen OSCC patients (mean age 55 ± 11 years; 69% male) and nineteen healthy controls (mean age 55 ± 10 years; 79% male) were analyzed. Distinct microbial profiles were observed in OSCC patients, including pathogenic species previously associated with carcinogenesis, suggesting potential biomarkers for oral cancer.

Conclusions

The MALDI Biotyper is an effective, noninvasive tool for identifying salivary microbiota. Its application may support early diagnosis and prognosis of OSCC, reinforcing the significance of the oral microbiome in cancer etiology.

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使用MALDI-TOF质谱鉴定口腔鳞状细胞癌患者需氧唾液微生物：一项初步研究的初步结果

口腔鳞状细胞癌（OSCC）是一种多因素疾病，新的证据表明口腔微生物群与其发展有关。快速、无创的唾液微生物鉴定可能为口腔癌的诊断和预后提供新的见解。方法应用MALDI Biotyper对OSCC患者和健康人的唾液微生物群进行分析。培养唾液样本，使用UltrafleXtreme MALDI-TOF质谱仪根据蛋白质谱图进行微生物鉴定。结果13例OSCC患者(平均年龄55±11岁；69%男性)和19名健康对照者(平均年龄55±10岁；79%为男性)。在OSCC患者中观察到不同的微生物谱，包括先前与致癌有关的致病物种，提示口腔癌的潜在生物标志物。结论MALDI生物分型仪是一种有效的、无创的唾液微生物群鉴定工具。它的应用可能支持OSCC的早期诊断和预后，强化口腔微生物组在癌症病因学中的意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.