Comparative assessment of endotoxin levels in drinking water: bioluminescence inhibition and Limulus Amebocyte Lysate (LAL) testing

IF 3 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

International Journal of Environmental Science and Technology Pub Date : 2025-03-11 DOI:10.1007/s13762-025-06432-2

R. Dehghan, Kh. Piri, A. Abdoli

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引用次数: 0

Abstract

The Jajrood River serves as a crucial drinking water source for Tehran, Iran, necessitating regular monitoring of its pollution levels to safeguard public health. Endotoxin remains a significant pollutant of concern, adversely affecting water quality. This study evaluates two detection methods for endotoxin levels: traditional Limulus Amebocyte Lysate testing and a bioluminescence inhibition assay using Vibrio fischeri. The objective is to compare the efficiency and accuracy of these methods in assessing water quality in both the Jajrood River and a connected water treatment facility. The findings reveal that the Vibrio fischeri assay can detect endotoxin levels as low as < 1 EU/mL within 15 min, significantly faster than the 120 min required for Limulus Amebocyte Lysate testing. However, external environmental factors impacted the accuracy of the Vibrio fischeri results. Notably, strong correlations were observed between the two methods (r = 0.61 for the river; r = 0.46 for the treatment plant), underscoring the urgent need for enhanced monitoring strategies for drinking water in Tehran. This research highlights the potential of innovative detection methods to improve public health safety.

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饮用水中内毒素水平的比较评价：生物发光抑制和鲎试剂（LAL）检测

贾伊鲁德河是伊朗德黑兰的重要饮用水源，必须定期监测其污染水平，以保障公众健康。内毒素仍然是一种重要的污染物，对水质有不利影响。本研究评估了两种检测内毒素水平的方法：传统的鲎试剂检测和使用费氏弧菌的生物发光抑制法。目的是比较这些方法在评估Jajrood河和连接的水处理设施的水质方面的效率和准确性。结果表明，费氏弧菌法可以在15分钟内检测出低至1 EU/mL的内毒素水平，明显快于鲎试剂检测所需的120分钟。然而，外部环境因素影响了费氏弧菌检测结果的准确性。值得注意的是，两种方法之间存在很强的相关性(河流的r = 0.61；r = 0.46（处理厂），强调迫切需要加强对德黑兰饮用水的监测战略。这项研究突出了创新检测方法在改善公共卫生安全方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Environmental Science and Technology 环境科学-环境科学

CiteScore

5.60

自引率

6.50%

发文量

806

审稿时长

10.8 months

期刊介绍： International Journal of Environmental Science and Technology (IJEST) is an international scholarly refereed research journal which aims to promote the theory and practice of environmental science and technology, innovation, engineering and management. A broad outline of the journal''s scope includes: peer reviewed original research articles, case and technical reports, reviews and analyses papers, short communications and notes to the editor, in interdisciplinary information on the practice and status of research in environmental science and technology, both natural and man made. The main aspects of research areas include, but are not exclusive to; environmental chemistry and biology, environments pollution control and abatement technology, transport and fate of pollutants in the environment, concentrations and dispersion of wastes in air, water, and soil, point and non-point sources pollution, heavy metals and organic compounds in the environment, atmospheric pollutants and trace gases, solid and hazardous waste management; soil biodegradation and bioremediation of contaminated sites; environmental impact assessment, industrial ecology, ecological and human risk assessment; improved energy management and auditing efficiency and environmental standards and criteria.