Synthetic microbiome and biochar enhanced human feces aerobic mesophilic composting

IF 11.2 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Resources Conservation and Recycling Pub Date : 2025-05-05 DOI:10.1016/j.resconrec.2025.108342

Xiaoyu Cai , Jing Wang , Kun Feng , Huihui Zhou , Chuan Chen , Chuandong Wu , Rui Fang , Defeng Xing

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引用次数: 0

Abstract

Effective methods to enhance aerobic composting of human feces are essential for improving composting efficiency and mitigating nitrogen loss. In this study, the effects of a synthetic human feces microbiome (SHFM) and biochar on human feces aerobic composting were investigated. Compared with the control group, the composting system inoculated with 10 % SHFM and biochar demonstrated the highest removal efficiencies for total solids, organics and nitrogen retention rate, with the enhancements of 29.86 ± 0.72 %, 16.15 ± 1.19 %, and 9.82 ± 0.42 %, respectively. Microbiome analysis revealed that Bacillus and Streptococcus from SHFM successfully colonized in the human fecal composting system. Pseudomonas, Nonomuraea, and Rhizobium which could enhance organics removal and nitrogen retention were enriched in the samples inoculated with SHFM and biochar. Moreover, the inoculation of SHFM and biochar increased complexities and the number of keystone species in species co-occurrence networks, indicating the enhancement of network stability. These findings suggested that the synergistic effect of synthetic microbiome and biochar represented an ideal strategy for improving human feces aerobic composting.

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合成微生物组和生物炭增强人类粪便好氧中温堆肥

提高粪便的好氧堆肥效率和减少氮的损失至关重要。本研究研究了合成人类粪便微生物组（SHFM）和生物炭对人类粪便好氧堆肥的影响。与对照组相比，添加10% SHFM和生物炭的堆肥体系对总固形物、有机物和氮的去除效率最高，分别提高了29.86±0.72%、16.15±1.19%和9.82±0.42%。微生物组分析显示，来自SHFM的芽孢杆菌和链球菌在人类粪便堆肥系统中成功定植。在接种了SHFM和生物炭的样品中富集了增强有机物去除和氮保留的假单胞菌、单胞菌和根瘤菌。此外，接种SHFM和生物炭增加了物种共生网络的复杂性和关键物种数量，表明网络稳定性增强。这些结果表明，合成微生物组和生物炭的协同效应是改善人类粪便好氧堆肥的理想策略。

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来源期刊

Resources Conservation and Recycling 环境科学-工程：环境

CiteScore

22.90

自引率

6.10%

发文量

625

审稿时长

23 days

期刊介绍： The journal Resources, Conservation & Recycling welcomes contributions from research, which consider sustainable management and conservation of resources. The journal prioritizes understanding the transformation processes crucial for transitioning toward more sustainable production and consumption systems. It highlights technological, economic, institutional, and policy aspects related to specific resource management practices such as conservation, recycling, and resource substitution, as well as broader strategies like improving resource productivity and restructuring production and consumption patterns. Contributions may address regional, national, or international scales and can range from individual resources or technologies to entire sectors or systems. Authors are encouraged to explore scientific and methodological issues alongside practical, environmental, and economic implications. However, manuscripts focusing solely on laboratory experiments without discussing their broader implications will not be considered for publication in the journal.