Chemical composition and in vitro intestinal probiotic properties analysis of exopolysaccharides from screened Streptococcus thermophilus 90301

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-05-02 DOI:10.1016/j.ijbiomac.2025.143882

Xiaochan Wang , Tao Lin , Yihan Lin , Chang Xu , Kai Ma , Changliang Zhang , Feng Ji , Ghahvechi Chaeipeima Mahsa , Xin Rui , Wei Li

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Abstract

A strain of Streptococcus thermophilus 90301, characterized by robust growth and high exopolysaccharide (EPS) production, was selected. Following isolation and purification, two EPS components, EPS-1 and EPS-2, were obtained. The analysis revealed that EPS-1 was composed of mannose, rhamnose, glucosamine, glucose, and galactose with molar ratios of 0.94:0.09:0.11:1.00:0.16, whereas EPS-2 was composed of mannose, ribose, glucose, and galactose with molar ratios of 15.11:0.26:1.00:0.38; respectively. The average molecular weights were determined to be 2.31 × 10⁵ Da for EPS-1 and 2.41 × 10⁵ Da for EPS-2. Furthermore, the prebiotic potential of the two fractions (EPS-1 and EPS-2) was investigated through in vitro simulation of human fecal fermentation digestion. Both EPS-1 and EPS-2 were not digested by the digestive fluids and were able to significantly modulate the composition of the gut microbiota. Additionally, they enhanced the gut microbiota's ability to produce short-chain fatty acids. These findings provide some basis for the functional application of polysaccharide components extracted from S. thermophilus 90301 as a potential prebiotic.

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嗜热链球菌90301体外多糖的化学组成及体外肠道益生菌特性分析

选择了一株生长旺盛、胞外多糖（EPS）产量高的嗜热链球菌90301。分离纯化得到EPS-1和EPS-2两个EPS组分。结果表明，EPS-1由甘露糖、鼠李糖、葡萄糖胺、葡萄糖和半乳糖组成，摩尔比为0.94:0.09:0.11:1.00:0.16；EPS-2由甘露糖、核糖、葡萄糖和半乳糖组成，摩尔比为15.11:0.26:1.00:0.38；分别。EPS-1的平均分子量为2.31 × 105 Da， EPS-2的平均分子量为2.41 × 105 Da。此外，通过体外模拟人体粪便发酵消化，研究了EPS-1和EPS-2两个组分的益生元潜力。EPS-1和EPS-2均不被消化液消化，并能显著调节肠道微生物群的组成。此外，它们还增强了肠道微生物群产生短链脂肪酸的能力。这些研究结果为嗜热链球菌90301多糖组分作为潜在益生元的功能应用提供了一定的依据。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.