Development of quantum dot-based tetrodotoxin immunochromatographic test strips and the effect of different aquatic product matrices on detection results

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Control Pub Date : 2025-04-29 DOI:10.1016/j.foodcont.2025.111400

Xinyi Zhao , Jinmin Zhang , Wenye Zhu , Hong Lin , Yaqing Wang , Zhenxing Li

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Abstract

Tetrodotoxin (TTX) is a highly potent marine neurotoxin that can cause fatal poisoning. It is found not only in pufferfish but also in other marine organisms such as shellfish. In recent years, the number of poisoning incidents due to the accidental consumption of TTX-containing aquatic products has increased. Therefore, the development of a rapid and sensitive TTX assay is urgently needed. In this study, quantum dot-based fluorescent lateral flow immunochromatographic test strips (LFICS) were developed, and the effects of different aquatic matrices on the assay were investigated. The fluorescent probe was synthesized by coupling a monoclonal antibody against TTX with carbonylated quantum dots, and the conditions for strip preparation were optimized. The results of the developed test strips could be observed within 15 min, with a visual detection limit (VDL) of 500 ng/mL and an instrumental detection limit (IDL) of 8.7 ng/mL. The linear range of detection (DLR) was 5–1000 ng/mL. The intra-assay and inter-assay coefficients of variation for the LFICS were 2.15 %–13.71 % and 2.7 %–14.55 %, respectively. Five aquatic samples (pomfret, cod, whelk, clam, and South American white shrimp) spiked with TTX were analyzed using the developed LFICS. The average recoveries ranged from 76 % to 96 % at spiked concentrations of 100 ng/mL and 1000 ng/mL, and from 47.97 % to 94.87 % at a spiked concentration of 10 ng/mL.

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基于量子点的河豚毒素免疫层析试纸条的研制及不同水产品基质对检测结果的影响

河豚毒素（TTX）是一种强效的海洋神经毒素，可引起致命中毒。它不仅存在于河豚中，也存在于其他海洋生物中，如贝类。近年来，因误食含ttx的水产品而导致的中毒事件有所增加。因此，迫切需要开发一种快速、灵敏的TTX检测方法。本研究研制了基于量子点的荧光侧流免疫层析试纸条（LFICS），并研究了不同水生基质对检测的影响。通过羰基化量子点偶联TTX单克隆抗体合成荧光探针，并对条带制备条件进行优化。所建立的试纸可在15 min内观察到结果，视觉检出限（VDL）为500 ng/mL，仪器检出限（IDL）为8.7 ng/mL。线性检测范围（DLR）为5 ~ 1000 ng/mL。LFICS的组内变异系数为2.15% ~ 13.71%，组间变异系数为2.7% ~ 14.55%。使用开发的LFICS对5种水生样品（鲳鱼、鳕鱼、海螺、蛤蜊和南美白虾）进行了TTX加标分析。加标浓度为100ng /mL和1000ng /mL时，平均加标回收率为76% ~ 96%；加标浓度为10ng /mL时，平均加标回收率为47.97% ~ 94.87%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Control 工程技术-食品科技

CiteScore

12.20

自引率

6.70%

发文量

758

审稿时长

33 days

期刊介绍： Food Control is an international journal that provides essential information for those involved in food safety and process control. Food Control covers the below areas that relate to food process control or to food safety of human foods: • Microbial food safety and antimicrobial systems • Mycotoxins • Hazard analysis, HACCP and food safety objectives • Risk assessment, including microbial and chemical hazards • Quality assurance • Good manufacturing practices • Food process systems design and control • Food Packaging technology and materials in contact with foods • Rapid methods of analysis and detection, including sensor technology • Codes of practice, legislation and international harmonization • Consumer issues • Education, training and research needs. The scope of Food Control is comprehensive and includes original research papers, authoritative reviews, short communications, comment articles that report on new developments in food control, and position papers.