Soft-hard self-alternating flexible organic-inorganic intercalated short-fiber mimetic bone lamellae

IF 12.7 1区材料科学 Q1 ENGINEERING, MULTIDISCIPLINARY

Composites Part B: Engineering Pub Date : 2025-04-28 DOI:10.1016/j.compositesb.2025.112581

Mingyue Liu , Xiaoyu Han , Guilai Zuo , Pengcheng Xiao , Yue Zhao , Xiumei Mo , Juan Wang , Wenguo Cui

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引用次数: 0

Abstract

Bone lamellae are the fundamental basis of bone structure and function. Simulating the multilevel ordered soft-hard alternating multilayer microstructures of these materials is extremely challenging. In this study, amorphous and highly structurally connected flexible inorganic silica nanofibers (SiO₂ NF) consisting of a network of silica-oxygen-silica bonds were prepared by sol-gel electrospinning and high-temperature calcination techniques, and highly entangled with organosodium alginate and hydroxyapatite nanoparticles (HAPs) to form soft-hard alternating structures wrapped in fixed-points by hydrogen bonding. Finally, soft-hard self-alternating flexible organic-inorganic intercalated short-fiber mimetic bone lamellae (ASH) were successfully constructed via the selective crystallization technique to adjust the temperature gradient for crystallization, thereby precipitating the intercalated structure. Based on the principles of crystal growth kinetics and solubility product equilibrium, ASH was transformed from a conventional disordered structure to a highly ordered multilayered soft-hard alternating structure with a porosity of up to 95 %. The ASH scaffold demonstrated exceptional shape memory properties, maintaining structural stability under 80 % strain and over 100 compression cycles. In vitro analyses revealed that sustained release of bioactive ions from ASH significantly enhanced osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), as evidenced by upregulated expression of Fgf and Pdf/Flt4 genes. Furthermore, in vivo studies validated the scaffold's capacity to promote BMSCs recruitment and migration, thereby accelerating bone regeneration. In summary, mimetic bone lamellae were successfully constructed and accurately replicated the microstructure of natural bone lamellae, providing a new perspective and strategy for exploring the structure-function relationships of bone lamellae.

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软硬自交变柔性有机-无机插层短纤维模拟骨片

骨板是骨结构和功能的基础。模拟这些材料的多级有序软硬交替多层微观结构是极具挑战性的。本研究采用溶胶-凝胶静电纺丝和高温煅烧技术制备了由二氧化硅-氧-二氧化硅键组成的无定形、高结构连接的柔性无机二氧化硅纳米纤维（SiO2 NF），并与有机海藻酸钠和羟基磷灰石纳米颗粒（HAPs）高度缠结，形成由氢键包裹在定点上的软硬交替结构。最后，通过选择结晶技术调节结晶温度梯度，成功构建软硬自交柔性有机-无机插层短纤维模拟骨片（ASH），从而形成插层结构。基于晶体生长动力学和溶解度生成物平衡原理，ASH从传统的无序结构转变为孔隙率高达95%的高度有序多层软硬交替结构。ASH支架表现出优异的形状记忆性能，在80%的应变和超过100次的压缩循环下保持结构稳定性。体外分析显示，ASH持续释放的生物活性离子显著增强骨髓间充质干细胞（BMSCs）的成骨分化，Fgf和Pdf/Flt4基因的表达上调证明了这一点。此外，体内研究证实了支架促进骨髓间充质干细胞募集和迁移的能力，从而加速骨再生。综上所述，我们成功构建并准确复制了天然骨片的微观结构，为探索骨片的结构-功能关系提供了新的视角和策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Composites Part B: Engineering 工程技术-材料科学：复合

CiteScore

24.40

自引率

11.50%

发文量

784

审稿时长

21 days

期刊介绍： Composites Part B: Engineering is a journal that publishes impactful research of high quality on composite materials. This research is supported by fundamental mechanics and materials science and engineering approaches. The targeted research can cover a wide range of length scales, ranging from nano to micro and meso, and even to the full product and structure level. The journal specifically focuses on engineering applications that involve high performance composites. These applications can range from low volume and high cost to high volume and low cost composite development. The main goal of the journal is to provide a platform for the prompt publication of original and high quality research. The emphasis is on design, development, modeling, validation, and manufacturing of engineering details and concepts. The journal welcomes both basic research papers and proposals for review articles. Authors are encouraged to address challenges across various application areas. These areas include, but are not limited to, aerospace, automotive, and other surface transportation. The journal also covers energy-related applications, with a focus on renewable energy. Other application areas include infrastructure, off-shore and maritime projects, health care technology, and recreational products.