Carbon metabolism and partitioning in citrus leaves is determined by hybrid, cultivar and leaf type

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-05-02 DOI:10.1016/j.plaphy.2025.109978

Mingjiong Zhao , Zhenshan Liu , Yanping Hu , Shilai Yi , Yueqiang Zhang , Bin Hu , Xiaojun Shi , Heinz Rennenberg

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Abstract

The partitioning and metabolism of carbohydrates and lignin in leaves are essential for numerous physiological functions, growth and development of plants. This study was aimed to characterize these processes in four leaf types (i.e., autumn-, summer-, spring- and current-year spring shoots) of two citrus hybrids (loose-skin mandarin cultivars OP (i.e., cultivars ‘Orah’ (OR) Citrus reticulata Blanco and ‘Ponkan’ (PO) Citrus reticulata Blanco and the sweet orange cultivars NT ‘Newhall navel orange’ (NO) Citrus sinensis (L.) Osbeck and ‘Tarocco’ (TA) Citrus sinensis (L.) Osbeck) differing in fruit maturation under field conditions. For this purpose, we analyzed the levels of foliar structural, non-structural carbohydrates and lignin and the expression of related genes. Our results showed that the contents of structural, non-structural carbohydrates and lignin measured in the two hybrids and its partitioning were mostly determined by differences in gene expression recorded in hybrids, cultivars and leaf type. Particularly, differences between leaf types were largely attributed to up- and down-regulation of the expression of genes of cellulose synthesis, lignin precursor synthesis, the Calvin cycle, glycolysis, the tricarbonic acid and starch synthesis and degradation pathways. These differences between leaf types required more complex transcriptional regulation than differences between hybrids and cultivars. The present results indicated that the two citrus hybrids studied differed in the expression of structural, non-structural carbohydrates and lignin-related genes. Future studies have to show if the differences observed in foliar partitioning and metabolism of carbohydrates and lignin are translated into partitioning and metabolism of carbohydrates and lignin in the roots.

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柑橘叶片碳代谢和分配受杂交品种、品种和叶片类型的影响

叶片中碳水化合物和木质素的分配和代谢对植物的许多生理功能和生长发育至关重要。本研究旨在对两种柑橘杂交品种（松皮柑橘品种OP（即‘ Orah ’ （OR） citrus reticulata Blanco和‘ Ponkan ’ （PO） citrus reticulata Blanco）和甜橙品种NT ‘ Newhall脐橙’ （NO） citrus sinensis (L.)）的四种叶片类型（即秋、夏、春、当年春芽）进行表征。Osbeck and ' Tarocco ' (TA) Citrus sinensis （L.）Osbeck)在田间条件下果实成熟不同。为此，我们分析了叶面结构碳水化合物、非结构碳水化合物和木质素的水平以及相关基因的表达。结果表明，结构碳水化合物、非结构碳水化合物和木质素的含量及其分配主要由杂交、品种和叶型的基因表达差异决定。特别是，不同叶型的差异很大程度上归因于纤维素合成、木质素前体合成、卡尔文循环、糖酵解、三碳酸和淀粉合成和降解途径基因表达的上调和下调。这些叶片类型之间的差异比杂种和品种之间的差异需要更复杂的转录调控。结果表明，两种柑橘杂种在结构碳水化合物、非结构碳水化合物和木质素相关基因的表达上存在差异。未来的研究必须表明，在叶片中观察到的碳水化合物和木质素的分配和代谢的差异是否转化为根中碳水化合物和木质素的分配和代谢。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.