Accelerated sludge granulation of novel complete ammonium and nitrate removal via denitratation anammox over nitrite process at elevated loading rates

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-04-30 DOI:10.1016/j.biortech.2025.132610

Ziyi Cheng , Jinyan Wang , Xinping Liu , Shenbin Cao

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Abstract

The Complete Ammonium and Nitrate Removal via Denitratation Anammox Over Nitrite (CANDAN) process was evaluated for rapid sludge granulation in a lab-scale sequencing batch reactor. Over 119 days under increasing nitrogen loading rates (NLRs), the system finally achieved average 89.2 % total nitrogen removal at 1.93 kg N/m³/d NLR, with sludge particle sizes increasing from 215.6 μm to 924.5 μm. Higher NLRs significantly increased extracellular polymeric substances, especially hydrophobic proteins, enhancing sludge hydrophobicity and aggregation. Metagenomic analysis identified Candidatus Brocadia and Thauera as predominant and key microbial genera for nitrogen removal. Furthermore, the upregulation of carbon metabolism under heightened NLRs facilitated the synthesis of hydrophobic amino acids, promoting sludge granulation. These findings demonstrate NLR-driven granulation mechanisms, highlight optimizing NLR as key for accelerating granulation, providing insights to improve start-up and operational efficiency of CANDAN systems.

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在高负荷速率下，通过亚硝酸盐反硝化厌氧氨氧化工艺加速新型完全去除铵硝的污泥造粒

在实验室规模的序批式反应器中，对亚硝酸盐厌氧氨氧化脱硝（CANDAN）工艺进行了快速污泥造粒的评价。在提高氮负荷率（NLRs）的条件下，经过119天的试验，系统最终以1.93 kg N/m3/d的NLR实现了平均89.2%的总氮去除率，污泥粒径从215.6 μm增加到924.5 μm。高nlr显著增加细胞外聚合物质，特别是疏水蛋白，增强污泥的疏水性和聚集性。宏基因组分析发现Brocadia和Thauera是脱氮的优势菌属和关键菌属。此外，高NLRs下碳代谢的上调促进了疏水氨基酸的合成，促进污泥造粒。这些发现证明了NLR驱动的造粒机制，突出了优化NLR是加速造粒的关键，为提高canan系统的启动和运行效率提供了见解。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.