Cargo Delivery to Cells Using Laser-Irradiated Carbon-Black-Loaded Polydimethylsiloxane

IF 9.6 1区化学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

ACS Materials Letters Pub Date : 2025-04-04 DOI:10.1021/acsmaterialslett.4c0245710.1021/acsmaterialslett.4c02457

Weilu Shen*, Anqi Chen, Gurminder K. Paink, Nicole Black, David A. Weitz and Eric Mazur,

引用次数: 0

Abstract

Effective intracellular delivery is essential for successful gene editing of cells. Spatially selective delivery to cells that is simultaneously precise, consistent, and nondestructive remains challenging using conventional state-of-the-art techniques. Here, we introduce a carrier-free method for spatiotemporal delivery of fluorescently labeled cargo into both adherent and suspension cells using carbon-black-embedded polydimethylsiloxane (PDMS) substrates irradiated by nanosecond laser pulses. This low-cost, biocompatible material, coupled with an optical approach, enables scalable, spatially selective, and sequential delivery of multiple cargo molecules, including FITC-Dextran and siRNA, to a broad range of cells. Notably, we achieved siRNA delivery into the cytoplasm of hard-to-transfect K562 cells with 45% efficiency, while maintaining nearly 100% cell viability.

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利用激光辐照炭黑负载聚二甲基硅氧烷向细胞输送货物

有效的细胞内递送是细胞基因编辑成功的必要条件。同时精确、一致和无损地对细胞进行空间选择性递送仍然是传统最先进技术的挑战。在这里，我们介绍了一种无载体的方法，利用纳秒激光脉冲照射下的碳黑嵌入聚二甲基硅氧烷（PDMS）衬底，将荧光标记的货物时空递送到贴壁和悬浮细胞中。这种低成本的生物相容性材料，加上光学方法，可以实现可扩展，空间选择性和顺序递送多种货物分子，包括fitc -葡聚糖和siRNA，到广泛的细胞中。值得注意的是，我们以45%的效率将siRNA传递到难以转染的K562细胞的细胞质中，同时保持了近100%的细胞活力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Materials Letters MATERIALS SCIENCE, MULTIDISCIPLINARY-

CiteScore

14.60

自引率

3.50%

发文量

261

期刊介绍： ACS Materials Letters is a journal that publishes high-quality and urgent papers at the forefront of fundamental and applied research in the field of materials science. It aims to bridge the gap between materials and other disciplines such as chemistry, engineering, and biology. The journal encourages multidisciplinary and innovative research that addresses global challenges. Papers submitted to ACS Materials Letters should clearly demonstrate the need for rapid disclosure of key results. The journal is interested in various areas including the design, synthesis, characterization, and evaluation of emerging materials, understanding the relationships between structure, property, and performance, as well as developing materials for applications in energy, environment, biomedical, electronics, and catalysis. The journal has a 2-year impact factor of 11.4 and is dedicated to publishing transformative materials research with fast processing times. The editors and staff of ACS Materials Letters actively participate in major scientific conferences and engage closely with readers and authors. The journal also maintains an active presence on social media to provide authors with greater visibility.