Chemical and biological assessment of biosynthesized Magnetite (Fe3O4) nanoparticles (NPs) mediated quercetin extracted from Allium cepa

Abstract

Bioactive phytochemicals derived from plant extracts have drawn considerable interest due to their potential for the creation of novel medications and for the ‘environmentally friendly’ synthesis of ‘nanoparticles.’ In the present study, a variety of techniques, including LC-Mass, NMR, and CHNOS elements, were used to identify quercetin, which was extracted from chopped onion and later utilized as a reducing agent for the “biosynthesis of Fe3O4 nanoparticles”. The obtained Fe₃O₄ was characterized by zeta potential (Z-P), field emission scanning electron microscopy, EDs, TEM, and zeta potentials. Air-dried chopped onion (2 kg) was extracted and purified, yielding 24.0 %. The structure of the isolated quercetin was determined using column chromatography, spectroscopic techniques, and elemental analysis. In this study, Fe₃O₄ nanoparticles were prepared using quercetin extract. The antiviral and antibacterial activities of Quercetin, and Fe₃O₄ NPs were determined using the well diffusion method against pathogenic microbes, Staphylococcus aureus and Escherichia coli. It was observed that the greatest effect against influenza virus and bacterial strains was Quercetin combined with Fe₃O₄ nanoparticles compared with Quercetin and Fe³O₄ NPs alone. Our findings suggest that Fe₃O₄ NPs, and Quercetin may be used in the future as a synergistic model for more biomedical applications.