Exogenous coumarin improves cell wall and plasma membrane stability and function by maintaining copper and calcium homeostasis in citrus roots under copper excess

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-04-29 DOI:10.1016/j.plaphy.2025.109949

Wei-Lin Huang , Wei-Tao Huang , Xu-Feng Chen , Ti Wu , Liang-Yuan Tong , Tian-Tian Xia , Bi-Sha Wu , Fei Lu , Ning-Wei Lai , Lin-Tong Yang , Li-Song Chen

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引用次数: 0

Abstract

Most citrus trees are planted in acidic soil with high availability of copper (Cu). Little is known about the mechanisms by which coumarin (COU) reduces Cu excess in plants. ‘Xuegan’ (Citrus sinensis) seedlings were treated with 0.5 (Cu0.5) or 400 (Cu excess or Cu400) CuCl₂ and 0 (COU0) or 100 (COU100) μM COU for 24 weeks. COU100 alleviated Cu400-induced alterations in gene expression and metabolite profiles, cell wall (CW) materials (CWMs), CW components (CWCs), and Fourier transform infrared (FTIR) spectra of CWMs in roots; increase in Cu concentration in roots, root CWMs (RCWMs), root CWCs (RCWCs), Cu and Ca fractions in RCWMs, and Cu fraction in CW pectin; and decrease in Ca concentrations in roots, RCWMs, and RCWCs. In addition, COU100 mitigated Cu400-induced increase in electrolyte leakage and concentrations of total coumarins, total phenolics, total falvonoids, and nonstructural carbohydrates (NCs) and decrease in total free amino acid concentration in roots, as well as impairment in root system architecture (RSA) and root growth. Our results corroborated the hypothesis that the alleviation of root Cu excess by COU was caused by the combination of following several aspects: (a) reduced impairment to root growth and RSA; (b) upregulated ability to maintain CW and plasma membrane stability and function by maintaining Cu and calcium homeostasis; (c) elevated adaptability of primary metabolism to Cu excess; and (d) upregulated biosynthesis and catabolism (turnover) of secondary metabolites (SMs) and less upregulation of SMs. COU0-treated roots underwent some physiological and molecular adaptations to Cu excess.

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外源香豆素通过维持柑橘根铜和钙的稳态，改善了铜过量条件下柑橘根细胞壁和质膜的稳定性和功能

大多数柑橘树种植在含铜量高的酸性土壤中。关于香豆素（COU）减少植物中过量铜的机制，人们知之甚少。用0.5 （Cu0.5）或400 (Cu过量或Cu400) CuCl2和0 （COU0）或100 (COU100) μM COU处理雪柑幼苗24周。COU100减轻了cu400诱导的根内CWMs基因表达、代谢谱、细胞壁物质、组分和FTIR光谱的改变；根、根CWMs （RCWMs）、根CWCs （RCWCs）、根CWMs中的Cu和Ca组分以及CW果胶中的Cu组分均增加；根系、RCWMs和RCWCs中Ca浓度降低。此外，COU100还减轻了cu400诱导的电解质泄漏、总香豆素、总酚类、总黄酮类和非结构性碳水化合物（nc）浓度增加、根中总游离氨基酸浓度降低以及根系结构（RSA）和根生长的损害。我们的研究结果证实了COU减轻根Cu过量的假设是由以下几个方面的综合作用引起的：(a)减少了对根生长和RSA的损害；(b)通过维持Cu和钙稳态来维持CW和质膜稳定性和功能的能力上调；(c)初级代谢对Cu过量的适应性提高；(d)次级代谢物（SMs）的生物合成和分解代谢（周转）上调，而SMs的上调较少。co0处理的根对Cu过量表现出一定的生理和分子适应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.