Transformation of the drug ibuprofen by Priestia megaterium: reversible glycosylation and generation of hydroxylated metabolites

IF 5.8 3区 环境科学与生态学 0 ENVIRONMENTAL SCIENCES
Tjorven Hinzke, Rabea Schlüter, Annett Mikolasch, Daniela Zühlke, Patrick Müller, Robert Kleditz, Katharina Riedel, Michael Lalk, Dörte Becher, Halah Sheikhany, Frieder Schauer
{"title":"Transformation of the drug ibuprofen by Priestia megaterium: reversible glycosylation and generation of hydroxylated metabolites","authors":"Tjorven Hinzke,&nbsp;Rabea Schlüter,&nbsp;Annett Mikolasch,&nbsp;Daniela Zühlke,&nbsp;Patrick Müller,&nbsp;Robert Kleditz,&nbsp;Katharina Riedel,&nbsp;Michael Lalk,&nbsp;Dörte Becher,&nbsp;Halah Sheikhany,&nbsp;Frieder Schauer","doi":"10.1007/s11356-025-36393-5","DOIUrl":null,"url":null,"abstract":"<div><p>As one of the most-consumed drugs worldwide, ibuprofen (IBU) reaches the environment in considerable amounts as environmental pollutant, necessitating studies of its biotransformation as potential removal mechanism. Here, we screened bacteria with known capabilities to degrade aromatic environmental pollutants, belonging to the genera <i>Bacillus</i>, <i>Priestia</i> (formerly also <i>Bacillus</i>), <i>Paenibacillus</i>, <i>Mycobacterium</i>, and <i>Cupriavidus</i>, for their ability to transform ibuprofen. We identified seven transformation products, namely 2-hydroxyibuprofen, carboxyibuprofen, ibuprofen pyranoside, 2-hydroxyibuprofen pyranoside, 4-carboxy-α-methylbenzene-acetic acid, 1-[4-(2-hydroxy-2-methylpropyl)phenyl]ethanone, and 2-hydroxyibuprofenmethyl ester. Based on our screening results, we focused on ibuprofen biotransformation by <i>Priestia megaterium</i> SBUG 518, to identify structures of transformation products, and to shed light on the drug’s impact on bacterial physiology. Biotransformation reactions by <i>P. megaterium</i> SBUG 518 involved (A) the hydroxylation of the isobutyl side chain at two positions, and (B) conjugate formation via esterification with a sugar molecule of the carboxylic group of ibuprofen and an ibuprofen hydroxylation product. Glycosylation seems to be a detoxification process, since the ibuprofen conjugate (ibuprofen pyranoside) was considerably less toxic than the parent compound to <i>P. megaterium</i> SBUG 518. Based on proteome profile changes and inhibition assays, cytochrome P450 systems appear to be central for ibuprofen transformation in <i>P. megaterium</i> SBUG 518. The toxic effect of ibuprofen appears to be caused by interference of the drug with different physiological pathways, especially sporulation.</p></div>","PeriodicalId":545,"journal":{"name":"Environmental Science and Pollution Research","volume":"32 19","pages":"11981 - 11995"},"PeriodicalIF":5.8000,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s11356-025-36393-5.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Science and Pollution Research","FirstCategoryId":"93","ListUrlMain":"https://link.springer.com/article/10.1007/s11356-025-36393-5","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"0","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

As one of the most-consumed drugs worldwide, ibuprofen (IBU) reaches the environment in considerable amounts as environmental pollutant, necessitating studies of its biotransformation as potential removal mechanism. Here, we screened bacteria with known capabilities to degrade aromatic environmental pollutants, belonging to the genera Bacillus, Priestia (formerly also Bacillus), Paenibacillus, Mycobacterium, and Cupriavidus, for their ability to transform ibuprofen. We identified seven transformation products, namely 2-hydroxyibuprofen, carboxyibuprofen, ibuprofen pyranoside, 2-hydroxyibuprofen pyranoside, 4-carboxy-α-methylbenzene-acetic acid, 1-[4-(2-hydroxy-2-methylpropyl)phenyl]ethanone, and 2-hydroxyibuprofenmethyl ester. Based on our screening results, we focused on ibuprofen biotransformation by Priestia megaterium SBUG 518, to identify structures of transformation products, and to shed light on the drug’s impact on bacterial physiology. Biotransformation reactions by P. megaterium SBUG 518 involved (A) the hydroxylation of the isobutyl side chain at two positions, and (B) conjugate formation via esterification with a sugar molecule of the carboxylic group of ibuprofen and an ibuprofen hydroxylation product. Glycosylation seems to be a detoxification process, since the ibuprofen conjugate (ibuprofen pyranoside) was considerably less toxic than the parent compound to P. megaterium SBUG 518. Based on proteome profile changes and inhibition assays, cytochrome P450 systems appear to be central for ibuprofen transformation in P. megaterium SBUG 518. The toxic effect of ibuprofen appears to be caused by interference of the drug with different physiological pathways, especially sporulation.

布洛芬药物在大孢子虫体内的转化:可逆糖基化和羟基化代谢物的生成
布洛芬(ibuprofen, IBU)是世界上使用量最大的药物之一,它作为环境污染物大量进入环境,因此有必要对其生物转化作为潜在的去除机制进行研究。在这里,我们筛选了已知具有降解芳香环境污染物能力的细菌,属于芽孢杆菌属,Priestia(以前也称为芽孢杆菌),Paenibacillus, Mycobacterium和Cupriavidus,因为它们具有转化布洛芬的能力。我们鉴定了7个转化产物,分别是2-羟基布洛芬、羧基布洛芬、布洛芬吡喃苷、2-羟基布洛芬吡喃苷、4-羧基-α-甲基苯乙酸、1-[4-(2-羟基-2-甲基丙基)苯基]乙酮和2-羟基布洛芬甲酯。基于我们的筛选结果,我们将重点关注布洛芬在Priestia megaterium SBUG 518中的生物转化,以确定转化产物的结构,并阐明药物对细菌生理的影响。megaterium SBUG 518的生物转化反应包括(A)异丁基侧链两个位置的羟基化,以及(B)与布洛芬羧基的糖分子和布洛芬羟基化产物的酯化偶联反应。糖基化似乎是一个解毒过程,因为布洛芬偶联物(布洛芬吡喃苷)比母体化合物对megaterium sbug518的毒性要小得多。基于蛋白质组谱的变化和抑制实验,细胞色素P450系统似乎是巨芽孢杆菌sbug518中布洛芬转化的核心。布洛芬的毒性作用似乎是由于药物干扰了不同的生理途径,特别是孢子的产生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
8.70
自引率
17.20%
发文量
6549
审稿时长
3.8 months
期刊介绍: Environmental Science and Pollution Research (ESPR) serves the international community in all areas of Environmental Science and related subjects with emphasis on chemical compounds. This includes: - Terrestrial Biology and Ecology - Aquatic Biology and Ecology - Atmospheric Chemistry - Environmental Microbiology/Biobased Energy Sources - Phytoremediation and Ecosystem Restoration - Environmental Analyses and Monitoring - Assessment of Risks and Interactions of Pollutants in the Environment - Conservation Biology and Sustainable Agriculture - Impact of Chemicals/Pollutants on Human and Animal Health It reports from a broad interdisciplinary outlook.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信