Limited in vivo conversion of dietary D3 to 25-OH-D3 in rainbow trout and undetectable endogenous photo-production of D3 in indoor-raised fish

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-04-29 DOI:10.1016/j.aquaculture.2025.742645

S. Rider , E. Chenal , B. Guy , P. Cabo-Valcarce , C. Chatelle , A. Schattner , C. Modugno , S. Etheve , E. Santigosa

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引用次数: 0

Abstract

Vitamin D is essential for salmonids and is routinely supplemented in feeds as cholecalciferol (D₃). As an intermediate metabolite in the in vivo conversion of D₃ to active vitamin D (1,25-OH-D₃), directly adding calcifediol (25-OH-D₃) to feed may improve vitamin D status.

An 84-day feeding trial was conducted in rainbow trout (Oncorhynchus mykiss) raised indoors, to compare dietary 25-OH-D₃ and D₃ at levels of 200, 400, or 800 μg/kg. Zootechnical performance and the profiles of vitamin D metabolites in plasma, muscle, and liver were assessed.

D₃ and 25-OH-D₃ appeared in plasma, muscle, and liver in a dose-dependent manner. Both metabolites supported optimal zootechnical performance and maintained vitamin D status at all inclusion levels, as indicated by body weight, feed conversion, specific growth rate, and plasma 1,25-OH-D₃ concentrations. Plasma and muscle 25-OH-D₃ profiles showed that the conversion of dietary D₃ to 25-OH-D₃ in vivo was limited. Additionally, undetectable levels of D₃ in fish that did not receive this metabolite in their feed indicated the lack of any significant endogenous photo-production of D₃.

This study demonstrates that 25-OH-D₃ can support growth in salmonids. While adding D₃ to fish feeds increases respective levels in the body, the conversion of D₃ to 25-OH-D₃ is limited. Therefore, to raise in vivo levels of 25-OH-D₃ above this threshold, it needs to be direclty added to the feed. Since salmonids raised indoors do not produce detectable D₃ endogenously, they rely on dietary sources to meet their vitamin D requirements.

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在虹鳟鱼中，饲料D3向25-OH-D3的体内转化有限，而在室内养殖的鱼类中，D3的内源性产光量无法检测到

维生素D是鲑鱼所必需的，通常作为胆钙化醇（D3）在饲料中补充。作为体内D3转化为活性维生素D （1,25- oh -D3）的中间代谢物，在饲料中直接添加钙化二醇（25-OH-D3）可改善维生素D状态。采用室内饲养的虹鳟鱼（Oncorhynchus mykiss）进行了为期84 d的饲养试验，比较饲料中25-OH-D3和D3水平分别为200、400和800 μg/kg。评估了动物技术性能和血浆、肌肉和肝脏中维生素D代谢物的分布。D3和25-OH-D3以剂量依赖性方式出现在血浆、肌肉和肝脏中。通过体重、饲料转化率、特定生长率和血浆1,25- oh - d3浓度可以看出，这两种代谢物都支持最佳的动物技术性能，并在所有添加水平下维持维生素D状态。血浆和肌肉25-OH-D3谱显示，饲料中D3向体内25-OH-D3的转化是有限的。此外，在饲料中没有摄入这种代谢物的鱼体内检测不到D3水平，这表明缺乏任何显著的内源性D3光生产。这项研究表明，25-OH-D3可以支持鲑鱼的生长。虽然在鱼饲料中添加D3可以提高体内相应水平，但D3转化为25-OH-D3的过程是有限的。因此，要将25-OH-D3的体内水平提高到该阈值以上，需要直接添加到饲料中。由于室内饲养的鲑鱼不能内源性产生可检测到的维生素D3，它们依靠饮食来源来满足维生素D的需求。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.