Differential regulation of antioxidant machinery on grass pea partial resistance against powdery mildew and rust pathogens

IF 2.8 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-04-26 DOI:10.1016/j.pmpp.2025.102717

Davide Coelho Martins , Francisco A. Mendes , Susana de Sousa Araújo , Diego Rubiales , Maria Carlota Vaz Patto

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引用次数: 0

Abstract

Powdery mildew (Erysiphe pisi, E. trifolii) and rust (Uromyces pisi) are important diseases affecting grass pea (Lathyrus sativus). This study investigates grass pea's histological, enzymatic, and metabolic responses to these pathogens using accessions with contrasting resistance. Partially resistant (PR) accessions exhibited smaller fungal colonies from 48 h after inoculation (HAI) onwards. Enhanced superoxide dismutase (SOD) activity was observed as early as 12 HAI in PR accessions against both powdery mildews, associated with increased ascorbate peroxidase (APX) and catalase (CAT) activity in E. trifolii and E. pisi infections, respectively. Moreover, phenolic compounds and flavonoids accumulated in E. trifolii-infected PR accessions (6–48 HAI). For rust, APX activity rose at 48 HAI in PR accessions.

These findings suggest that partial resistance (PR) in grass pea is characterized by restricted pathogen invasion and a dynamic regulation of reactive oxygen species (ROS)-scavenging enzymes, with responses varying across pathosystems. This highlights the importance of pathogen-specific selection strategies to minimize the risk of resistance breakdown and promote durable disease resistance in breeding programs.

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草豆抗氧化机制对白粉病和锈病部分抗性的差异调控

草豆白粉病（Erysiphe pisi， E. trifolii）和锈病（Uromyces pisi）是影响草豆的重要病害。本研究研究了草豆对这些病原体的组织学、酶和代谢反应，使用具有对比抗性的材料。部分抗性（PR）材料在接种后48 h真菌菌落减小。早在12个HAI时，两种白粉病对PR材料的超氧化物歧化酶（SOD）活性均有增强，这与三叶杆菌和尿囊杆菌感染时抗坏血酸过氧化物酶（APX）和过氧化氢酶（CAT）活性的增加有关。此外，酚类化合物和黄酮类化合物在三叶杆菌感染的PR材料中积累（6-48 HAI）。对于锈病，APX活性在PR材料中上升至48 HAI。这些发现表明，草豆的部分抗性（PR）以病原体入侵受限和活性氧（ROS）清除酶的动态调节为特征，在不同的病理系统中反应不同。这突出了病原特异性选择策略的重要性，以尽量减少抗性崩溃的风险，并促进育种计划中持久的抗病能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.