Maturity phase is crucial for removing antibiotic resistance genes during composting: novel insights into dissolved organic matter-microbial symbiosis system

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-04-30 DOI:10.1016/j.biortech.2025.132607

Bing Bai , Lixia Wang , Fachun Guan , Houan Pi , Anxun Wang , Limei Zhai

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Abstract

Composting is widely regarded as an effective method for reducing antibiotic resistance genes (ARGs) in livestock and poultry manure. However, the critical mechanisms of ARGs in different composting phase are still unclear. In this study, normal composting and two types of rapid composting (without mature phase) were used to analyze the removal of ARGs and the succession of dissolved organic matter (DOM). Compared to normal composting, rapid composting reactivated tetracyclines, sulfonamide, and quinolones resistance genes during the maturation phase and reduced the total ARGs removal rates by 45.58 %–57.87 %. Humus-like components could inhibit the proliferation of ARGs, and the enrichment of protein-like components increased abundances of Pusillimonas, Persicitalea, and Pseudomonas, indirectly reducing the removal. This study is the first to demonstrate the contribution of DOM and microbial community to ARGs removal, emphasizing the importance of the maturation phase for ARGs elimination. This research provides guidance for producing safe compost products.

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成熟期是在堆肥过程中去除抗生素抗性基因的关键：对溶解有机物-微生物共生系统的新见解

堆肥被广泛认为是减少畜禽粪便中抗生素抗性基因（ARGs）的有效方法。然而，在不同的堆肥阶段，ARGs的关键机制尚不清楚。本研究采用常规堆肥和两种快速堆肥（无成熟期），分析了ARGs的去除和溶解有机物（DOM）的演为。与普通堆肥相比，快速堆肥在成熟阶段重新激活了四环素类、磺胺类和喹诺酮类耐药基因，使总ARGs去除率降低了45.58% ~ 57.87%。腐殖质样组分可以抑制ARGs的增殖，蛋白样组分的富集增加了Pusillimonas、Persicitalea和Pseudomonas的丰度，间接降低了ARGs的去除。本研究首次证明了DOM和微生物群落对ARGs去除的贡献，强调了成熟阶段对ARGs去除的重要性。本研究为安全生产堆肥产品提供了指导。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.