Influence of titanium dioxide nanotubes on macrophage polarization and endothelial cell vascularisation under oxidative stress microenvironment

IF 4.7 3区材料科学 Q2 CHEMISTRY, PHYSICAL

Colloid and Interface Science Communications Pub Date : 2025-05-02 DOI:10.1016/j.colcom.2025.100840

Xinpeng Liu , Dini Lin , Shan Peng , Ronghua Yu , Bailong Tao , Lin Du , Hong Zheng , Xinkun Shen , Yonglin Yu

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Abstract

Elderly fracture healing is significantly impaired by oxidative stress-induced vascular dysfunction. This study investigates the effects of 110 nm titanium dioxide nanotubes (TNT₁₁₀) on macrophage polarization and endothelial cell vascularization under oxidative stress. Under H₂O₂-induced oxidative stress, RAW264.7 macrophages cultured on TNT₁₁₀ exhibit enhanced M1 polarization, with significantly upregulated M1 marker expression versus the Ti group. Conditioned medium from TNT₁₁₀-stimulated macrophages markedly promoted HUVEC migration and tube formation by activating the ERK/Akt signaling pathway. In vivo, TNT₁₁₀ implants demonstrate superior neovascularization (CD31⁺ areas) and bone regeneration compared to pure titanium. These findings suggest that TNT₁₁₀ enhances vascular and bone tissue regeneration under oxidative stress by modulating macrophage polarization and endothelial cell signaling pathways.

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氧化应激微环境下二氧化钛纳米管对巨噬细胞极化和内皮细胞血管化的影响

氧化应激诱导的血管功能障碍明显损害老年人骨折愈合。本文研究了氧化应激下110 nm二氧化钛纳米管（TNT110）对巨噬细胞极化和内皮细胞血管化的影响。在h2o2诱导的氧化应激下，TNT110培养的RAW264.7巨噬细胞表现出增强的M1极化，与Ti组相比，M1标记物表达显著上调。tnt110刺激巨噬细胞的条件培养基通过激活ERK/Akt信号通路，显著促进HUVEC迁移和小管形成。在体内，与纯钛相比，TNT110植入物表现出更好的新生血管（CD31+区域）和骨再生。这些发现表明，TNT110通过调节巨噬细胞极化和内皮细胞信号通路，促进氧化应激下血管和骨组织的再生。

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来源期刊

Colloid and Interface Science Communications Materials Science-Materials Chemistry

CiteScore

9.40

自引率

6.70%

发文量

125

审稿时长

43 days

期刊介绍： Colloid and Interface Science Communications provides a forum for the highest visibility and rapid publication of short initial reports on new fundamental concepts, research findings, and topical applications at the forefront of the increasingly interdisciplinary area of colloid and interface science.