The new strategies for high efficiency removal of antibiotics and antibiotic resistance genes by direct bio-drying of biogas slurry: Microbiological mechanisms

IF 11.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2025-05-01 DOI:10.1016/j.watres.2025.123763

Zhenye Tong , Haorong Zhang , Siqin Li , Liqian Ma , Zhenguo Li , Xiaoyu Yong , Fenwu Liu , Jun Zhou

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Abstract

High levels of antibiotics and antibiotic resistance genes (ARGs) still exist in biogas slurry after anaerobic digestion of cow manure. In this study, direct bio-drying strategies of cow manure biogas slurry without solid-liquid separation for the removal of antibiotics and ARGs were explored. The results showed that, after direct bio-drying of biogas slurry, the moisture contents decreased to 25.2 %–31.5 %. The maximum temperatures of the piles reached 76.1–77.4 °C, which is close to ultra-high temperatures (>80 °C). Direct biogas slurry bio-drying (CK treatment) achieved efficient removal of antibiotics, ARGs, and mobile genetic elements (MGEs) (95.4 %, 98.6 % and 86.7 % removal, respectively). Compared to the CK treatment, molecular membrane covering (MMC) alone was the most effective in further significantly decreasing the antibiotic concentration and the abundance of ARGs and MGEs in the final bio-dried samples, followed by food waste hydrochar (FHC) addition alone. Methanogenic archaea were identified as potential hosts for ARGs based on Network analysis. FHC addition–MMC increased the abundance of potential hosts for ARGs and promoted the expression of microbial methane metabolism function relative to the CK treatment during the later stages of bio-drying, thereby decreasing the removal efficiency of ARGs. The results of structural equation model and redundancy analysis showed that MGEs had the most significant direct effect on ARGs and moisture content had the highest relative contribution to changes in ARGs. In summary, direct bio-drying strategies were able to efficiently remove antibiotics and ARGs from cow manure biogas slurry and also achieve biological dewatering of the biogas slurry.

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沼液直接生物干燥高效去除抗生素和耐药基因的新策略：微生物机制

牛粪厌氧消化后的沼液中仍存在高水平的抗生素和抗生素耐药基因（ARGs）。本研究探讨了牛粪沼液不经固液分离直接生物干燥去除抗生素和ARGs的策略。结果表明，沼液经直接生物干燥后，含水率降至25.2% ~ 31.5%。桩的最高温度达到76.1 ~ 77.4℃，接近超高温（>80℃）。直接沼液生物干燥（CK处理）对抗生素、ARGs和移动遗传因子（MGEs）的去除率分别为95.4%、98.6%和86.7%。与CK处理相比，单用分子膜覆盖（MMC）处理在进一步显著降低最终生物干燥样品中抗生素浓度及ARGs和MGEs丰度方面效果最好，其次是单用厨余氢炭（FHC）处理。通过网络分析，确定产甲烷古菌为ARGs的潜在寄主。在生物干燥后期，相对于CK处理，添加FHC - mmc增加了ARGs潜在寄主的丰度，促进了微生物甲烷代谢功能的表达，从而降低了ARGs的去除效率。结构方程模型和冗余分析结果表明，MGEs对ARGs的直接影响最为显著，含水率对ARGs变化的相对贡献最大。综上所述，直接生物干燥策略可以有效去除牛粪沼液中的抗生素和ARGs，并实现沼液的生物脱水。

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来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.