Simultaneous Cd immobilization and oxidative stress alleviation in Brassica chinensis by a novel phosphate-solubilizing strain Sutcliffiella horikoshii P1

Abstract

Microbial remediation of cadmium (Cd) pollution offers economically green and operationally simple advantages, particularly in environments with mild contamination. The acquisition of efficient strains and coupling between bacterial response and plant fitness are current research emphases in the remediation process. In this study, a novel phosphate-solubilizing strain with outstanding Cd-resistance, Sutcliffiella (S.) horikoshii P1 was isolated. Cd removal efficiency reached 98.85 % by the strain within 24 h at an initial concentration of 5 mg/L. Distribution analysis revealed that the dominant mechanism of Cd removal by the strain varies with Cd concentrations. Notably, the seed soaking of Brassica chinensis with S. horikoshii P1 could improve seed germination rate and growth potential regardless of the presence of Cd stress. Morphological and biochemical trait analysis revealed that the inoculated strain also increased fresh weight and reduced the Cd phytoavailability of Brassica chinensis by producing active substances and alleviating plant oxidative stress. Pot experiment demonstrated that the transport factor (TF) and bioconcentration factor (BCF) decreased by 22.76 % and 33.59 %, respectively, and Cd content in edible parts met food safety standards. Whole-genome sequencing analysis demonstrated that functional genes related to heavy metal resistance and transport (cadC, czcD, znuA, etc.), and gene clusters involved in siderophore secretion may regulate Cd immobilization and the plant growth-promoting effect of S. horikoshii P1. The results underscored the feasibility and effectiveness of Sutcliffiella horikoshii in addressing Cd contamination and promoting plant growth, providing a basis for the future application in agricultural safe production.