Adsorption and release profile analysis of oxytetracycline drug on functionalized graphene oxide nanoparticles

Abstract

In this study graphite particles were converted to graphene oxide (GO) via Hummer's method. Synthesized graphene oxide was used for studying adsorption and release of oxytetracycline in dog's simulated gastric. This material the synthesized GO was functionalized with epichlorohydrin and Cibacron blue. Due to the relatively high-water solubility of oxytetracycline, it can be easily dissolved in body fluids without sufficient absorption and storage time. Drugs which are soluble in water, easily dissolve in body fluids and won't store in body well enough according to their ease of solubility in water. Therefore, the drug is likely to be eliminated via kidney and urinary system without adequate adsorption and satisfactory therapeutic effect. won't have convenient effect. In order to overcome the rapid elimination of oxytetracycline prolonged drug release was considered. Oxytetracycline is an antibiotic. antibiotics are generally linked with bacterial ribosomal unit and thus inhibit protein synthesis in bacteria. In this study optimum pH for drug was investigated firstly and according to results obtain acidic environment has the best adsorption for the studied drug. First, the optimal pH value for the drug was investigated. Based on the results obtained the acidic environment provided the best adsorption conditions for oxytetracycline. According to studies dog's digestive system is acidic and drugs and food remains in it for approximately 8 h. Experiments demonstrated that oxytetracycline GO nanoparticles show the highest drug release in the first 2 h making the investigation of drug release in the intestines unnecessary. Finally, the accuracy of synthesis, adsorption and release were investigated by UV–Vis experiments. The obtained adsorption isotherm fits Langmuir and Temkin models well, and the theoretical maximum of adsorption capacity calculated by Langmuir model is 495 mg/g.The adsorption capacities of tetracycline oxytetracycline on GO decreased with increasing pH. The adsorption isotherms of oxytetracycline on graphene oxide were discussed and compared. The aim of this study is to make and modify graphene oxide in order to make a pharmaceutical adsorbent for the slow adsorption and release of the drug oxytetracycline in the simulated environment of the dog's body and the release of oxytetracycline was modified and enhanced by epichlorohydrin and Cibacron Blue and was investigated.