Restoration of gut integrity by Bacteroides acidifaciens in water-deprived conditions

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical and biophysical research communications Pub Date : 2025-04-28 DOI:10.1016/j.bbrc.2025.151917

Siyuan Fan , Xinyue Cai , Wenbo Cui , Peisen Ma , Meiling Wu , Jing Guo , Yipeng Zhang , Kun Xuan , Zihan Li

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引用次数: 0

Abstract

Water scarcity exerts profound physiological impacts, yet its effects on intestinal microbiota-barrier crosstalk remain poorly understood. This study investigates water-deprivation-induced gut dysbiosis and identifies Bacteroides acidifaciens (B.acidifaciens) as a critical mediator of epithelial barrier resilience. Using a murine model of graded water restriction, we observed colon shortening, villus atrophy, goblet cell loss, and barrier disruption correlated with water-deprivation severity. Oral supplementation of B.acidifaciens restored colonic architecture, enhanced mucin secretion, and ameliorated barrier dysfunction in water-deprived mice. Mechanistically, B.acidifaciens maintained barrier homeostasis by directly stimulating mucus production in intestinal epithelial cells and upregulating the expression of tight junction proteins. These findings establish B.acidifaciens as a critical species in mitigating water-deprivation-induced colon injury and advance our understanding of microbiota-directed interventions for dehydration-associated gastrointestinal disorders.

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缺水条件下酸化拟杆菌对肠道完整性的恢复作用

水资源短缺具有深远的生理影响，但其对肠道微生物群屏障串扰的影响尚不清楚。本研究调查了缺水引起的肠道生态失调，并确定了酸化拟杆菌（b.a ivacifaciens）是上皮屏障恢复能力的关键介质。使用分级限水小鼠模型，我们观察到结肠缩短、绒毛萎缩、杯状细胞丢失和屏障破坏与缺水严重程度相关。在缺水小鼠中，口服补充酸化芽胞杆菌可恢复结肠结构，增强粘蛋白分泌，改善屏障功能障碍。从机制上讲，酸化芽胞杆菌通过直接刺激肠上皮细胞粘液的产生和上调紧密连接蛋白的表达来维持屏障稳态。这些发现证实了酸化芽胞杆菌是缓解缺水引起的结肠损伤的关键物种，并促进了我们对微生物群干预脱水相关胃肠道疾病的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemical and biophysical research communications 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

1400

审稿时长

14 days

期刊介绍： Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination of timely and significant experimental results in diverse fields of biological research. The development of the "Breakthroughs and Views" section brings the minireview format to the journal, and issues often contain collections of special interest manuscripts. BBRC is published weekly (52 issues/year).Research Areas now include: Biochemistry; biophysics; cell biology; developmental biology; immunology ; molecular biology; neurobiology; plant biology and proteomics