Negative correlation between oil lipolysis and its LC-PUFA content in simulated digestion

IF 6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

LWT - Food Science and Technology Pub Date : 2025-04-23 DOI:10.1016/j.lwt.2025.117835

Gabriele Beltrame , Iida Valta, Annelie Damerau, Kaisa M. Linderborg

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引用次数: 0

Abstract

The growing awareness of the pro-oxidant conditions of the gastrointestinal tract promotes attention to the fate of n-3 PUFAs after ingestion. These investigations primarily rely on in vitro models, with INFOGEST 2.0 being the most commonly applied. However, scarce attention is currently paid to the hydrolysis degree of the oils. In the present short communication, lipid digesta of DHA-rich oils, obtained after in vitro digestion, were analyzed with NMR spectroscopy. Rapeseed oil was used as positive control. Results showed that the DHA content of the oil negatively affected lipolysis, with an observed negative non-linear relationship between lipolysis extent and the long chain n-3/n-6 fatty acid ratio of the oil. ¹³C NMR revealed a clear negative relationship between lipolysis and relative amount of 22:6n-3 in the sn-1,3 position. This intrinsic obstacle should be taken into account during the in vitro research on behavior of n-3-rich oils in the gastrointestinal tract.

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模拟消化过程中油脂分解与其LC-PUFA含量呈负相关

对胃肠道促氧化条件的日益认识促进了对摄入后n-3 PUFAs命运的关注。这些研究主要依赖于体外模型，其中最常用的是INFOGEST 2.0。然而，目前很少关注油脂的水解程度。本文用核磁共振光谱法对体外消化得到的富含dha的油脂进行了分析。以菜籽油为阳性对照。结果表明，鱼油中DHA含量对脂肪分解呈负向影响，脂肪分解程度与鱼油长链n-3/n-6脂肪酸比值呈负非线性关系。13C核磁共振显示，脂解与sn-1,3位22:6n-3的相对量呈明显负相关。在体外研究富n-3油脂在胃肠道中的行为时，应考虑到这一内在障碍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

LWT - Food Science and Technology 工程技术-食品科技

CiteScore

11.80

自引率

6.70%

发文量

1724

审稿时长

65 days

期刊介绍： LWT - Food Science and Technology is an international journal that publishes innovative papers in the fields of food chemistry, biochemistry, microbiology, technology and nutrition. The work described should be innovative either in the approach or in the methods used. The significance of the results either for the science community or for the food industry must also be specified. Contributions written in English are welcomed in the form of review articles, short reviews, research papers, and research notes. Papers featuring animal trials and cell cultures are outside the scope of the journal and will not be considered for publication.