Antibacterial effect of Vachellia farnesiana (L.) WIGHT & ARN. pod extract against gram positive and gram negative multidrug-resistant strains

Pharmacological Research - Natural Products Pub Date : 2025-04-27 DOI:10.1016/j.prenap.2025.100245

Denise Aline Casimiro Bezerra , Maria Anésia Sousa de Alencar , Dárcio Luiz de Sousa Júnior , Luciene Ferreira De Lima , Ítalo Mykaell da Silva Benjamim , Luiz Eduardo Oliveira Teotônio , Nadghia Figueiredo Leite Sampaio , Pedro Everson Alexandre De Aquino , Ítalo Rosal Lustosa , Joanda Paolla Raimundo e Silva , Josean Fechine Tavares , Marcelo Sobral da Silva , Micheline Azevedo Lima , Lucas Silva Abreu , Jaime Ribeiro-Filho , Francisco Assis Bezerra Da Cunha , Henrique Douglas Melo Coutinho

{"title":"Antibacterial effect of Vachellia farnesiana (L.) WIGHT & ARN. pod extract against gram positive and gram negative multidrug-resistant strains","authors":"Denise Aline Casimiro Bezerra , Maria Anésia Sousa de Alencar , Dárcio Luiz de Sousa Júnior , Luciene Ferreira De Lima , Ítalo Mykaell da Silva Benjamim , Luiz Eduardo Oliveira Teotônio , Nadghia Figueiredo Leite Sampaio , Pedro Everson Alexandre De Aquino , Ítalo Rosal Lustosa , Joanda Paolla Raimundo e Silva , Josean Fechine Tavares , Marcelo Sobral da Silva , Micheline Azevedo Lima , Lucas Silva Abreu , Jaime Ribeiro-Filho , Francisco Assis Bezerra Da Cunha , Henrique Douglas Melo Coutinho","doi":"10.1016/j.prenap.2025.100245","DOIUrl":null,"url":null,"abstract":"<div><div>Antibiotic resistance rates have increased over time, requiring the development of new antimicrobial drugs and the identification of bioactive compounds capable of improving the effectiveness of currently available antibiotics. <em>Vachellia farnesiana</em> (L.) is a species of the Fabaceae family characterized by phenolic compounds and terpenes. Thus, considering the evidence that these compounds have significant biological effects, this study aimed to characterize the chemical composition and antibacterial effects of the ethanolic extract of <em>V. farnesiana</em> pods against standard and multidrug-resistant (MDR) strains of <em>Staphylococcus aureus</em>, <em>Escherichia coli</em>, and <em>Pseudomonas aeruginosa</em>. The minimum inhibitory concentration (MIC) was determined to assess the antibacterial activity of the extract, as well as to investigate its enhancing the effectiveness of amikacin, gentamicin, and clindamycin against MDR strains. Therefore, the antibiotics were combined with a sub-inhibitory extract concentration (MIC/8). The extract showed significant antibacterial activity against <em>S. aureus</em> and <em>E. coli</em>. In addition, the association of antibiotics with a sub-inhibitory concentration (MIC/8) of the extract significantly reduced the MIC of at least two drugs against different strains, indicating potentiation. Finally, the HPLC-DAD-ESI-MS analyses detected the presence of 25 peaks, with the identification of 30 compounds in 22 peaks (88 %) in the extracts of <em>V. farnesiana</em> green pods.</div></div>","PeriodicalId":101014,"journal":{"name":"Pharmacological Research - Natural Products","volume":"7 ","pages":"Article 100245"},"PeriodicalIF":0.0000,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacological Research - Natural Products","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2950199725001053","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Antibiotic resistance rates have increased over time, requiring the development of new antimicrobial drugs and the identification of bioactive compounds capable of improving the effectiveness of currently available antibiotics. Vachellia farnesiana (L.) is a species of the Fabaceae family characterized by phenolic compounds and terpenes. Thus, considering the evidence that these compounds have significant biological effects, this study aimed to characterize the chemical composition and antibacterial effects of the ethanolic extract of V. farnesiana pods against standard and multidrug-resistant (MDR) strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The minimum inhibitory concentration (MIC) was determined to assess the antibacterial activity of the extract, as well as to investigate its enhancing the effectiveness of amikacin, gentamicin, and clindamycin against MDR strains. Therefore, the antibiotics were combined with a sub-inhibitory extract concentration (MIC/8). The extract showed significant antibacterial activity against S. aureus and E. coli. In addition, the association of antibiotics with a sub-inhibitory concentration (MIC/8) of the extract significantly reduced the MIC of at least two drugs against different strains, indicating potentiation. Finally, the HPLC-DAD-ESI-MS analyses detected the presence of 25 peaks, with the identification of 30 compounds in 22 peaks (88 %) in the extracts of V. farnesiana green pods.

查看原文本刊更多论文

金花莲子的抑菌作用怀特和阿恩。豆荚提取物抗革兰氏阳性和革兰氏阴性多重耐药菌株

抗生素耐药率随着时间的推移而增加，要求开发新的抗菌药物和鉴定能够提高现有抗生素有效性的生物活性化合物。豆科植物Vachellia farnesiana （L.）是一种以酚类化合物和萜烯为特征的植物。因此，考虑到这些化合物具有显著的生物学效应，本研究旨在表征法氏荚果乙醇提取物对金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌标准菌株和多药耐药菌株的化学成分和抑菌作用。测定其最低抑菌浓度（MIC），并考察其对阿米卡星、庆大霉素和克林霉素对耐多药菌株的增强作用。因此，抗生素与亚抑制提取物浓度（MIC/8）联合使用。提取物对金黄色葡萄球菌和大肠杆菌具有明显的抑菌活性。此外，抗生素与提取物的亚抑制浓度（MIC/8）的关联显著降低了至少两种药物对不同菌株的MIC，表明增强作用。最后，通过HPLC-DAD-ESI-MS分析，检测到25个峰的存在，鉴定出22个峰中的30个化合物（88 %）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Pharmacological Research - Natural Products

自引率

0.00%

发文量