Neuroprotective effects of intraperitoneally injected Mg alloy extracts on middle cerebral artery occluded mouse with reperfusion injury

IF 15.8 1区材料科学 Q1 METALLURGY & METALLURGICAL ENGINEERING

Journal of Magnesium and Alloys Pub Date : 2025-04-01 DOI:10.1016/j.jma.2024.05.025

Lanjing Wang , Yue Hu , Hongkang Zhang , Miaowen Jiang , Yang Zhang , Yumin Luo , Xunming Ji , Yufeng Zheng , Sijie Li , Ming Li

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引用次数: 0

Abstract

Magnesium (Mg) alloy stents have been commercialized for the treatment of cardiovascular diseases and extensively studied for possible implantation in major organs. However, the in vivo studies regarding the neuroactivity of Mg alloys extracts are few. Therefore, the safety and neuroprotective effects of released degradation products (Mg²⁺ and H₂) from Mg alloys on ischemic brain tissues were investigated. To be specific, C57BL/6 mice with middle cerebral artery occlusion (MCAO) were injected intraperitoneally of saline, pure Mg extracts, WE43 extracts and hydrogen (H₂)-removed WE43 Mg alloy extracts (75 mL/kg) respectively immediately after reperfusion. The neurobehavioral assessment stated clearly that WE43 extracts could remarkably recover the neurological deficits of MCAO mice. We explored the underlying mechanisms and demonstrated that the Mg²⁺ and H₂ from WE43 extracts could perform anti-inflammatory and anti-oxidative function after MCAO in mice, by increasing the expressions of IL-4, TGF-β1, SOD1 and decreasing the expressions of IL-1β, CXCR2, MDA. Simultaneously, Mg²⁺ and H₂ contributed to increase cerebral blood flow and restore blood-brain barrier integrity. In conclusion, this study provides certain theoretical representation of the application of Mg alloy stents in intracranial vessels.

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腹腔注射镁合金提取物对大脑中动脉再灌注损伤小鼠的神经保护作用

镁（Mg）合金支架已被商业化用于心血管疾病的治疗，并被广泛研究用于主要器官植入的可能性。然而，关于镁合金提取物的神经活性的体内研究却很少。因此，研究了镁合金释放的降解产物（Mg2+和H2）对缺血脑组织的安全性和神经保护作用。对大脑中动脉闭塞（MCAO）的C57BL/6小鼠，在再灌注后立即腹腔注射生理盐水、纯Mg提取物、WE43提取物和去氢WE43镁合金提取物（75 mL/kg）。神经行为学评价表明，WE43提取物对MCAO小鼠的神经功能缺损有明显的恢复作用。我们对其机制进行了探讨，发现WE43提取物中的Mg2+和H2通过增加IL-4、TGF-β1、SOD1的表达，降低IL-1β、CXCR2、MDA的表达，具有抗炎和抗氧化作用。同时，Mg2+和H2有助于增加脑血流量，恢复血脑屏障的完整性。总之，本研究为镁合金支架在颅内血管中的应用提供了一定的理论代表。

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来源期刊

Journal of Magnesium and Alloys Engineering-Mechanics of Materials

CiteScore

20.20

自引率

14.80%

发文量

审稿时长

59 days

期刊介绍： The Journal of Magnesium and Alloys serves as a global platform for both theoretical and experimental studies in magnesium science and engineering. It welcomes submissions investigating various scientific and engineering factors impacting the metallurgy, processing, microstructure, properties, and applications of magnesium and alloys. The journal covers all aspects of magnesium and alloy research, including raw materials, alloy casting, extrusion and deformation, corrosion and surface treatment, joining and machining, simulation and modeling, microstructure evolution and mechanical properties, new alloy development, magnesium-based composites, bio-materials and energy materials, applications, and recycling.