Gibberellin signaling regulates pectin biosynthesis in Arabidopsis

IF 14.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2025-04-30 DOI:10.1038/s41467-025-59268-2

Yan Xu, Jinge Du, Ruili Hao, Siqi Ma, Yujiao Ma, Gongke Zhou, Ruibo Hu, Shengjun Li

{"title":"Gibberellin signaling regulates pectin biosynthesis in Arabidopsis","authors":"Yan Xu, Jinge Du, Ruili Hao, Siqi Ma, Yujiao Ma, Gongke Zhou, Ruibo Hu, Shengjun Li","doi":"10.1038/s41467-025-59268-2","DOIUrl":null,"url":null,"abstract":"<p>Pectin is an abundant polysaccharide with essential roles in various biological processes. Despite considerable advances in understanding the regulatory mechanisms of pectin biosynthesis, the influence of phytohormones on this process remains unclear. Here we report that gibberellins (GA) promotes pectin biosynthesis in Arabidopsis. The DELLA proteins, as GA signaling repressors, interact with TRANSPARENT TESTA GLABRA2 (TTG2) and components of the MYB-bHLH-WD40 (MBW) complex, the key regulators of pectin biosynthesis, to repress their transcriptional regulatory activities. Furthermore, the MBW proteins and TTG2 physically interact and synergistically activate the downstream target <i>GLABRA2</i>, whereas this interaction and collaboration are competitively attenuated by DELLAs. Genetic analyses validate that GA-mediated pectin biosynthesis relies on functional TTG2 and MBW proteins. Moreover, the pectin biosynthesis mediated by the GA-DELLA-MBW-TTG2 module contributes to GA-regulated seedling growth. Our findings reveal the significance of the GA-DELLA-MBW-TTG2 signaling cascade in the regulation of pectin biosynthesis and plant development.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"20 1","pages":""},"PeriodicalIF":14.7000,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature Communications","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.1038/s41467-025-59268-2","RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Pectin is an abundant polysaccharide with essential roles in various biological processes. Despite considerable advances in understanding the regulatory mechanisms of pectin biosynthesis, the influence of phytohormones on this process remains unclear. Here we report that gibberellins (GA) promotes pectin biosynthesis in Arabidopsis. The DELLA proteins, as GA signaling repressors, interact with TRANSPARENT TESTA GLABRA2 (TTG2) and components of the MYB-bHLH-WD40 (MBW) complex, the key regulators of pectin biosynthesis, to repress their transcriptional regulatory activities. Furthermore, the MBW proteins and TTG2 physically interact and synergistically activate the downstream target GLABRA2, whereas this interaction and collaboration are competitively attenuated by DELLAs. Genetic analyses validate that GA-mediated pectin biosynthesis relies on functional TTG2 and MBW proteins. Moreover, the pectin biosynthesis mediated by the GA-DELLA-MBW-TTG2 module contributes to GA-regulated seedling growth. Our findings reveal the significance of the GA-DELLA-MBW-TTG2 signaling cascade in the regulation of pectin biosynthesis and plant development.

Abstract Image

查看原文本刊更多论文

赤霉素信号传导调节拟南芥果胶的生物合成

果胶是一种丰富的多糖，在多种生物过程中起着重要作用。尽管在了解果胶生物合成的调控机制方面取得了相当大的进展，但植物激素对这一过程的影响仍不清楚。本文报道了赤霉素（GA）促进拟南芥果胶的生物合成。DELLA蛋白作为GA信号抑制因子，与果胶生物合成的关键调控因子TRANSPARENT TESTA GLABRA2 （TTG2）和MYB-bHLH-WD40 （MBW）复合物的组分相互作用，抑制其转录调控活性。此外，MBW蛋白和TTG2物理相互作用并协同激活下游靶标GLABRA2，而这种相互作用和协作被della竞争性地减弱。遗传分析证实，ga介导的果胶生物合成依赖于功能TTG2和MBW蛋白。此外，GA-DELLA-MBW-TTG2模块介导的果胶生物合成有助于ga调控的幼苗生长。我们的研究结果揭示了GA-DELLA-MBW-TTG2信号级联在果胶生物合成和植物发育调控中的重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.