Two-mode ratiometric electrochemical and turn-on fluorescence strategy for detecting biothiols in serum with all-in-one molecular probe

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-04-29 DOI:10.1016/j.bioelechem.2025.108995

Hui Dong , Ke Xu , Zuying Wang , Wentian Chen , Yitong Jiang , Yidan Ye , Zhu Chang , Xu Zhu , Yanli Zhou , Maotian Xu , Yintang Zhang

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Abstract

Reactive sulfur species (RSS) play crucial roles in human physiology, with biothiols like hydrogen sulfide (H₂S), cysteine (Cys), and glutathione (GSH) being key regulators of oxidative stress and signal transduction. Here, a dual-mode resorufin-based (Re) small-molecule probe (Re-RSS) with electrochemical and fluorescent properties was developed for biothiol detection. Using a ratiometric electrochemical strategy and a “turn-on” fluorescence method, Re-RSS enabled sensitive quantification of H₂S, Cys, and GSH. The electrochemical approach showed linear ranges of 0–0.3 mM for H₂S, 0–0.1 mM for Cys, and 0–0.08 mM for GSH. Fluorescence spectrometry revealed linear relationships in the ranges of 1 μM–0.3 mM (H₂S), 2 μM–0.1 mM (Cys), and 5 μM–0.08 mM (GSH), with detection limits as low as 0.02, 0.01, and 0.08 μM (S/N = 3), respectively. This dual-mode sensor offers three major advantages: (1) dual-readout correction of system/background errors for improved accuracy; (2) independent signal transduction from distinct mechanisms, eliminating cross-interference; (3) negative-potential electrochemical detection free from biological interference. The direct dual-mode quantification highlights its significance and reliability for biothiol analysis in biological systems.

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一键式分子探针检测血清中生物硫醇的双模式比例电化学和开启荧光策略

活性硫物质（RSS）在人体生理中起着至关重要的作用，硫化氢（H2S）、半胱氨酸（Cys）和谷胱甘肽（GSH）等生物硫醇是氧化应激和信号转导的关键调节剂。本文研制了一种具有电化学和荧光特性的双模间苯二酚基（Re）小分子探针（Re- rss），用于生物硫醇检测。利用比例电化学策略和“开启”荧光方法，Re-RSS实现了H2S、Cys和GSH的敏感定量。电化学方法显示H2S的线性范围为0-0.3 mM， Cys为0-0.1 mM， GSH为0-0.08 mM。荧光光谱在1 μM - 0.3 mM （H2S）、2 μM - 0.1 mM （Cys）和5 μM - 0.08 mM （GSH）范围内呈线性关系，检出限分别低至0.02、0.01和0.08 μM （S/N = 3）。该双模传感器具有三个主要优点：(1)双读出校正系统/背景误差，提高精度；(2)不同机制的独立信号转导，消除交叉干扰；(3)不受生物干扰的负电位电化学检测。直接双模定量在生物系统中分析生物硫醇具有重要意义和可靠性。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.