Advances in supercritical fluid chromatography for lipid analysis and purification – A review of the past decade

IF 3.4 3区工程技术 Q2 CHEMISTRY, PHYSICAL

Journal of Supercritical Fluids Pub Date : 2025-04-23 DOI:10.1016/j.supflu.2025.106638

Lou Spanneut , Thibaut Devièse

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Abstract

This review explores the advances in Supercritical Fluid Chromatography (SFC) for the analysis and purification of lipids achieved over the past decade. Lipidomics has become an essential tool in numerous fields such as biology and food science. Compared to liquid and gas chromatography, SFC, which utilizes supercritical CO₂ as a mobile phase, offers faster analysis, reduced solvent use, and improved separation of complex lipidic substances. This review synthesizes findings from over 80 studies, evaluating various stationary phases, retention mechanisms, and optimized conditions for both lipid class and intra-class separations. It provides detailed comparisons of columns for different lipid types, such as fatty acids, glycerolipids, and phospholipids, and explores preparative-scale applications for bioactive lipid purification, including omega-3 fatty acids and sterols. By consolidating this knowledge, this review will serve as a practical guide for chemists to select optimal columns and conditions without extensive testing, thereby streamlining method development and reducing costs.

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超临界流体色谱法用于脂质分析和纯化的研究进展

本文综述了近十年来超临界流体色谱（SFC）在脂质分析和纯化方面取得的进展。脂质组学已成为生物学和食品科学等众多领域的重要工具。与液相和气相色谱法相比，SFC利用超临界二氧化碳作为流动相，分析速度更快，溶剂用量减少，复杂脂质物质的分离效果更好。本综述综合了80多项研究的结果，评估了不同的固定相，保留机制，并优化了脂类和类内分离的条件。它提供了不同的脂质类型，如脂肪酸，甘油脂和磷脂柱的详细比较，并探讨制备规模应用的生物活性脂质纯化，包括omega-3脂肪酸和甾醇。通过巩固这方面的知识，这篇综述将作为一个实用的指导，为化学家选择最佳的色谱和条件，而不需要广泛的测试，从而简化方法开发和降低成本。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Supercritical Fluids 工程技术-工程：化工

CiteScore

7.60

自引率

10.30%

发文量

236

审稿时长

56 days

期刊介绍： The Journal of Supercritical Fluids is an international journal devoted to the fundamental and applied aspects of supercritical fluids and processes. Its aim is to provide a focused platform for academic and industrial researchers to report their findings and to have ready access to the advances in this rapidly growing field. Its coverage is multidisciplinary and includes both basic and applied topics. Thermodynamics and phase equilibria, reaction kinetics and rate processes, thermal and transport properties, and all topics related to processing such as separations (extraction, fractionation, purification, chromatography) nucleation and impregnation are within the scope. Accounts of specific engineering applications such as those encountered in food, fuel, natural products, minerals, pharmaceuticals and polymer industries are included. Topics related to high pressure equipment design, analytical techniques, sensors, and process control methodologies are also within the scope of the journal.