Novel fluorescence and smartphone-assisted colorimetric sensing platform based on ionic liquid carbon dots for rapid detection of amylase in urine

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-04-29 DOI:10.1016/j.ijbiomac.2025.143654

Xiujuan Wang, Jiarui Wang, Haojia Tian, Chenzhao Wang, Xiaowen Qiu, Zhicong Li, Zhibing Wang

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引用次数: 0

Abstract

Changes in amylase (AMY) concentration in urine serve a vital function in monitoring human health. However, the development of non-destructive, sensitive, and real-time assays for AMY concentration still faces serious challenges. In this study, a novel fluorescence/smartphone imaging colorimetric dual-mode sensing platform based on ionic liquid carbon dots (IL-CDs) was designed for the first time for assessing AMY concentration in urine. The obtained IL-CDs exhibited high sensitivity, good selectivity, and excellent stability. AMY can significantly decrease the fluorescence intensity through static quenching, enabling direct AMY concentration monitoring. Significantly, the proposed method was extended to the measurement of AMY in the urine samples with good spiked recoveries (96.98–103.81 %) as well as high accuracy. Furthermore, a smartphone-dependent detection method was developed after exploring the association between IL-CDs (G/(R + B)) fluorescence intensity at different concentrations of AMY, which simplified the AMY detection process. Through the combination of fluorescence and smartphone imaging colorimetry, it not only eliminated the damage to the human body during the detection process, but also realized the direct detection of AMY. The present method provided a fast and reliable new idea for monitoring human health.

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基于离子液体碳点的新型荧光和智能手机辅助比色传感平台快速检测尿液中淀粉酶

尿液中淀粉酶（AMY）浓度的变化在监测人体健康方面起着至关重要的作用。然而，开发无损、灵敏、实时的AMY浓度检测方法仍然面临着严峻的挑战。在这项研究中，首次设计了一种基于离子液体碳点（IL-CDs）的荧光/智能手机成像比色双模传感平台，用于评估尿中AMY浓度。所得IL-CDs灵敏度高，选择性好，稳定性好。AMY可以通过静态猝灭显著降低荧光强度，实现对AMY浓度的直接监测。值得注意的是，该方法可扩展到尿样中AMY的测定，加标回收率（96.98 ~ 103.81%）好，准确度高。此外，在探索了不同浓度AMY下IL-CDs （G/(R + B)）荧光强度之间的关系后，开发了一种依赖智能手机的检测方法，简化了AMY的检测过程。通过荧光与智能手机成像比色法的结合，不仅消除了检测过程中对人体的伤害，而且实现了对AMY的直接检测。该方法为人体健康监测提供了一种快速、可靠的新思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.