Evaluating denitrification performance and microbial com-munities in sorghum stalk-based systems: Optimal conditions for nitrate removal

IF 5.9 1区农林科学 Q1 AGRONOMY

Agricultural Water Management Pub Date : 2025-04-30 DOI:10.1016/j.agwat.2025.109510

Zhilong Wang, Jun Xie, Guangjun Wang, Zhifei Li, Kai Zhang, Yun Xia, Jingjing Tian, Hongyan Li, Wenping Xie, Wangbao Gong

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引用次数: 0

Abstract

Biological denitrification has promise for the removal of nitrate (NO₃⁻-N) from water. In this study, the effects of different temperatures, hydraulic retention times (HRTs), and influent nitrate concentrations (INCs) on denitrification performance were determined in a reverse nitrification system with sorghum stalk (SS) in a flow field environment. The optimum temperature for denitrification in constructed wetlands is 30 °C, under which the NO₃^--N and total nitrogen removal rates were 75.46 ± 6.77 % and 69.85 ± 6.06 %, respectively. The optimal HRT and INC for the sorghum stalk-denitrification reactor (SS-DR) were 32 h and 50 mg/L, respectively. Under these conditions, NO₃^--N and total nitrogen removal rates of SS-DR were 95.71 ± 1.75 % and 93.46 ± 1.36 %, respectively. High-throughput sequencing analysis revealed that the dominant phyla in the constructed wetlands were Proteobacteria, Chloroflexi, Firmicutes, Acidobacteriota, Desulfobacterota, Nitrospirota, Actinobacteriota, and Bacteroidota. Most Proteobacteria were facultative and obligate anaerobic denitrifying bacteria ubiquitous in sludge. Bacteroidota plays an important role in nitrogen cycling and energy conversion in ecosystems and decomposes macromolecular organic matter such as protein, cellulose, and lipids. Firmicutes were involved in the denitrification and degradation of cellulose, which has hydrolysis and acidification effects on agricultural waste. These findings provide a reference for the use of SS as an additional carbon source to enhance denitrification in the treatment of aquaculture wastewater.

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评估高粱秸秆系统的反硝化性能和微生物群落：去除硝酸盐的最佳条件

生物反硝化技术有望去除水中的硝酸盐（NO3−-N）。在流场环境下，研究了不同温度、水力停留时间（HRTs）和进水硝酸盐浓度（INCs）对高粱秸秆反硝化系统反硝化性能的影响。人工湿地反硝化的最佳温度为30℃，该温度下NO3——N和总氮去除率分别为75.46 ± 6.77 %和69.85 ± 6.06 %。高粱秸秆反硝化反应器（SS-DR）的最佳HRT和INC分别为32 h和50 mg/L。在此条件下，SS-DR对NO3——N和总氮的去除率分别为95.71 ± 1.75 %和93.46 ± 1.36 %。高通量测序结果显示，人工湿地的优势菌门为变形菌门、绿柔菌门、厚壁菌门、酸杆菌门、脱硫菌门、亚硝基螺旋体门、放线菌门和拟杆菌门。大多数变形菌属是污泥中普遍存在的兼性和专性厌氧反硝化细菌。拟杆菌群在生态系统中的氮循环和能量转化中起着重要作用，并能分解蛋白质、纤维素和脂类等大分子有机物。厚壁菌门参与纤维素的反硝化和降解，对农业废弃物具有水解和酸化作用。这些研究结果为在水产养殖废水处理中利用SS作为附加碳源加强反硝化提供了参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Agricultural Water Management 农林科学-农艺学

CiteScore

12.10

自引率

14.90%

发文量

648

审稿时长

4.9 months

期刊介绍： Agricultural Water Management publishes papers of international significance relating to the science, economics, and policy of agricultural water management. In all cases, manuscripts must address implications and provide insight regarding agricultural water management.