Deciphering of electron transfer and microbial community of electrogenic oxygen reducing biofilms to sulfamethoxazole stress

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-04-28 DOI:10.1016/j.biortech.2025.132597

Qing Jiang , Xing Dong , Yang Liu , Xiaoyu Zhou , Guomeng Sun , Ke Shi , Yanlu Qiao , Hao Jiang , Yujie Feng

{"title":"Deciphering of electron transfer and microbial community of electrogenic oxygen reducing biofilms to sulfamethoxazole stress","authors":"Qing Jiang , Xing Dong , Yang Liu , Xiaoyu Zhou , Guomeng Sun , Ke Shi , Yanlu Qiao , Hao Jiang , Yujie Feng","doi":"10.1016/j.biortech.2025.132597","DOIUrl":null,"url":null,"abstract":"<div><div>This study first evaluated the sulfamethoxazole (SMX) effects on oxygen-reducing biocathodes in microbial fuel cells (MFCs). Low SMX (0.5 mg L<sup>–1</sup>) enhanced current density by 20 % via increased direct electron transfer and lower charge transfer resistance. High SMX (10–30 mg L<sup>–1</sup>) suppressed electrochemical performance. SMX preferentially bound protein-like EPS components over fulvic-like fractions, inducing sequential structural changes (1054 > 970 > 3464 > 2921 > 1643 > 1350 cm<sup>−1</sup>). SMX exposure reshaped microbial communities, enriching antibiotic-resistant genera (<em>Truepera</em>, <em>Nitrospira</em>, <em>Brevundimonas</em>, etc.). Network analysis revealed low SMX enhanced community complexity/stability, while high doses simplified biofilm structure. Functional genes for electron transfer, carbon metabolism and oxidative phosphorylation increased at 0.5 mg L<sup>–1</sup> SMX but decreased under high concentrations. Overall, this study elucidates the dual role of SMX in modulating oxygen-reducing biofilm composition, function, and capability, laying the groundwork for optimized application of MFC in treating SMX-contaminated wastewater.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"430 ","pages":"Article 132597"},"PeriodicalIF":9.7000,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresource Technology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0960852425005632","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURAL ENGINEERING","Score":null,"Total":0}

引用次数: 0

Abstract

This study first evaluated the sulfamethoxazole (SMX) effects on oxygen-reducing biocathodes in microbial fuel cells (MFCs). Low SMX (0.5 mg L^–1) enhanced current density by 20 % via increased direct electron transfer and lower charge transfer resistance. High SMX (10–30 mg L^–1) suppressed electrochemical performance. SMX preferentially bound protein-like EPS components over fulvic-like fractions, inducing sequential structural changes (1054 > 970 > 3464 > 2921 > 1643 > 1350 cm⁻¹). SMX exposure reshaped microbial communities, enriching antibiotic-resistant genera (Truepera, Nitrospira, Brevundimonas, etc.). Network analysis revealed low SMX enhanced community complexity/stability, while high doses simplified biofilm structure. Functional genes for electron transfer, carbon metabolism and oxidative phosphorylation increased at 0.5 mg L^–1 SMX but decreased under high concentrations. Overall, this study elucidates the dual role of SMX in modulating oxygen-reducing biofilm composition, function, and capability, laying the groundwork for optimized application of MFC in treating SMX-contaminated wastewater.

Abstract Image

查看原文本刊更多论文

电致氧生物膜在磺胺甲恶唑胁迫下的电子转移和微生物群落解析

本研究首先评估了磺胺甲恶唑（SMX）对微生物燃料电池（mfc）中氧还原生物阴极的影响。低SMX （0.5 mg L-1）通过增加直接电子转移和降低电荷转移电阻，使电流密度提高20%。高浓度SMX （10-30 mg L-1）抑制了电化学性能。SMX优先结合蛋白样EPS组分而不是fulvici样组分，诱导序列结构变化(1054 >；970比;3464比;2921比;1643比;1350厘米−1)。SMX暴露重塑了微生物群落，丰富了抗生素耐药属（Truepera, Nitrospira， Brevundimonas等）。网络分析表明，低剂量SMX增强了群落的复杂性/稳定性，而高剂量SMX简化了生物膜结构。电子传递、碳代谢和氧化磷酸化功能基因在0.5 mg L-1 SMX处理下增加，高浓度下减少。总体而言，本研究阐明了SMX在调节氧还原生物膜组成、功能和能力方面的双重作用，为MFC在处理SMX污染废水中的优化应用奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.