Associations between serum and seminal vitamin D levels and sperm parameters

IF 1.6 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynaecology Research Pub Date : 2025-04-29 DOI:10.1111/jog.16305

Goksun Ipek, Sezcan Mumusoglu, Gurkan Bozdag, Serdar Gunalp

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引用次数: 0

Abstract

Aim

To examine the association between serum/seminal plasma vitamin D levels and sperm parameters, the possible effects of serum/seminal vitamin D on sperm motility via seminal intracellular calcium measurement, and the antioxidant effect via malondialdehyde (MDA) measurement.

Methods

This prospective study was conducted on 87 male patients. Serum vitamin D levels of patients were evaluated and grouped based on their serum vitamin D levels: low (<50 nmol/L), normal (50–75 nmol/L), and high (>75 nmol/L). Sperm characteristics were evaluated. Seminal plasma vitamin D, intracellular calcium, and MDA concentrations were measured using Liquid Chromatography Mass Spectrometry (LC–MS/MS), High-performance liquid chromatography (HPLC), and fluorescence plate reader methods, respectively.

Results

Patients with normal serum vitamin D levels had higher total progressive motile sperm concentrations (14.0 vs. 8.5 vs. 4 million/mL, p = 0.037) and higher normal sperm morphology (6% vs. 4% vs. 3%, p = 0.020) compared to those with low and high levels. No correlations were found between serum vitamin D levels and seminal vitamin D (r = 0.052, p = 0.468), MDA (r = −0.048, p = 0.791), and intracellular calcium levels (r = 0.064, p = 0.929). Seminal intracellular calcium levels were significantly correlated with total (r = 0.241, p = 0.024) and progressive (r = 0.217, p = 0.043) sperm motility.

Conclusions

There were positive correlations between serum vitamin D levels, total progressive motile sperm concentration, and normal sperm morphology. However, serum vitamin D levels were not correlated with seminal vitamin D levels and showed no correlations with potential mechanisms of action, reflected by intracellular calcium and MDA concentrations. Conversely, intracellular calcium levels were positively correlated with both total and progressive sperm motility, suggesting that intracellular calcium plays an essential role in sperm motility.

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血清和精液维生素D水平与精子参数之间的关系

目的探讨血清/精浆维生素D水平与精子参数的关系，通过精子胞内钙测定血清/精浆维生素D对精子活力的可能影响，以及通过丙二醛（MDA）测定血清/精浆维生素D的抗氧化作用。方法对87例男性患者进行前瞻性研究。评估患者血清维生素D水平，并根据血清维生素D水平分为低（50 nmol/L）、正常（50 - 75 nmol/L）和高（75 nmol/L）。评估精子特征。分别采用液相色谱-质谱法（LC-MS /MS）、高效液相色谱法（HPLC）和荧光板法测定精浆维生素D、细胞内钙和丙二醛（MDA）浓度。结果血清维生素D水平正常的患者与血清维生素D水平低和高的患者相比，总进动精子浓度（14.0比8.5比400万/mL， p = 0.037）和正常精子形态（6%比4%比3%，p = 0.020）更高。血清维生素D水平与精液维生素D （r = 0.052, p = 0.468）、MDA （r = - 0.048, p = 0.791）、细胞内钙水平（r = 0.064, p = 0.929）无相关性。精子细胞内钙水平与总精子活力（r = 0.241, p = 0.024）和进展精子活力（r = 0.217, p = 0.043）显著相关。结论血清维生素D水平、总进行性活动精子浓度与正常精子形态呈正相关。然而，血清维生素D水平与精液维生素D水平无关，也与细胞内钙和MDA浓度反映的潜在作用机制无关。相反，细胞内钙水平与精子总活力和进行性活力均呈正相关，表明细胞内钙在精子活力中起重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Obstetrics and Gynaecology Research 医学-妇产科学

CiteScore

3.10

自引率

0.00%

发文量

376

审稿时长

3-6 weeks

期刊介绍： The Journal of Obstetrics and Gynaecology Research is the official Journal of the Asia and Oceania Federation of Obstetrics and Gynecology and of the Japan Society of Obstetrics and Gynecology, and aims to provide a medium for the publication of articles in the fields of obstetrics and gynecology. The Journal publishes original research articles, case reports, review articles and letters to the editor. The Journal will give publication priority to original research articles over case reports. Accepted papers become the exclusive licence of the Journal. Manuscripts are peer reviewed by at least two referees and/or Associate Editors expert in the field of the submitted paper.