Ameliorative effects of chitosan on fluoride-induced kidney injury in rats: a stereological and immunohistochemical study

IF 2.9 4区生物学 Q3 CELL BIOLOGY

Journal of Molecular Histology Pub Date : 2025-04-30 DOI:10.1007/s10735-025-10428-0

Fikret Altındağ, Uğur Özdek

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引用次数: 0

Abstract

The present study aimed to investigate the possible protective effects of chitosan (CS) on fluoride-induced nephrotoxicity. 28 rats were divided into four groups (n = 7). The Control group received drinking water. Sodium fluoride (NaF) group received 100 mg/L NaF in drinking water. NaF + CS group received 100 mg/L NaF and 250 mg/kg/day CS by gastric gavage. CS group was given 250 mg/kg/day CS by gavage. The study period lasted 12 weeks. Total kidney volume, Bowman’s capsule volume, Bowman’s space volume, Tubular volume and Glomerulus volume were measured by stereological methods. Immunohistochemically, caspase-3 and TNF-alpha expressions were evaluated. Biochemically, levels of urea and creatinine were measured. In addition, a histopathological evaluation of the kidney was performed. According to the control group, an increase was observed in all stereological parameters except glomerulus volume in the NaF group. CS treatment inhibited the increase in stereological parameters. Fluoride increased expressions of caspase-3 and TNF-α in the kidney, and serum urea and creatine levels, but CS decreased these parameters. In addition, pathological changes in the kidney caused by fluoride such as tubular dilatation, enlargement of the Bowman’s space, and deterioration in tubular epithelial cells were restored with CS treatment. The conclusions of the current study reveal that fluoride can cause nephrotoxicity and CS treatment can prevent fluoride-induced nephrotoxicity.

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壳聚糖对氟化物所致大鼠肾损伤的改善作用：体视学和免疫组织化学研究

本研究旨在探讨壳聚糖（CS）对氟化物所致肾毒性可能的保护作用。28只大鼠分为4组（n = 7）。对照组给予饮水。氟化钠组饮水中添加氟化钠100 mg/L。NaF + CS组给予100 mg/L NaF和250 mg/kg/d CS灌胃。CS组给予CS 250 mg/kg/d灌胃。研究周期为12周。用体视学方法测定肾总体积、包囊体积、包囊空间体积、肾小管体积和肾小球体积。免疫组织化学检测caspase-3和tnf - α的表达。生化方面，测定尿素和肌酐水平。此外，对肾脏进行组织病理学评估。与对照组相比，NaF组除肾小球体积外，所有体视参数均增加。CS处理抑制了体视参数的增加。氟增加了肾脏中caspase-3和TNF-α的表达以及血清尿素和肌酸水平，但CS降低了这些参数。此外，氟引起的肾小管扩张、鲍曼间隙增大、肾小管上皮细胞退化等病理改变在CS处理后得到恢复。本研究结论表明氟可引起肾毒性，CS治疗可预防氟引起的肾毒性。

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.