Determination of Phenolics, Flavonoids, and Radical Scavenging Activity in Different Potato Tissues and In Vitro Callus Cultures of Punjab Potato-101, Kufri Pukhraj, and Lady Rosetta

IF 2.6 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2024-12-23 DOI:10.1007/s12161-024-02745-z

Pooja Manchanda, Harleen Kaur, Aiswarya V. Dev, Ramandeep Kaur Mankoo, Anu Kalia, Satpal Sharma

{"title":"Determination of Phenolics, Flavonoids, and Radical Scavenging Activity in Different Potato Tissues and In Vitro Callus Cultures of Punjab Potato-101, Kufri Pukhraj, and Lady Rosetta","authors":"Pooja Manchanda, Harleen Kaur, Aiswarya V. Dev, Ramandeep Kaur Mankoo, Anu Kalia, Satpal Sharma","doi":"10.1007/s12161-024-02745-z","DOIUrl":null,"url":null,"abstract":"<div><p>Potato (<i>Solanum tubersosum</i> L.) is the fourth largest food crop worldwide and is rich source of minerals, essential amino acids, vitamins, and other bioactive compounds. The present study was conducted to optimize extraction conditions for achieving maximum yield of TPC, TFC, and antioxidant potential from ethanolic extracts of tubers, whole plant parts, and callus cultures in Punjab Potato-101, Kufri Pukhraj, and Lady Rosetta. Callus cultures were obtained from nodal and internodal segments of potato explants. MS medium supplemented with 2,4-D (4.0 mg L<sup>−1</sup>) + kinetin (0.5 mg L<sup>−1</sup>) was found to be the best medium for callus induction. The results revealed that extraction using ethanol concentration of 40% at 50 °C for 45 min was the best for extraction of TPC, TFC from tubers, whole plant parts, and callus culture in all three cultivars. The same extraction conditions also yielded maximum amount of total sugars, amino acids, and vitamin C from callus cultures of all the three cultivars. Of the three cultivars, Lady Rosetta had maximum amount of TPC, TFC, and RSA from all the plant parts and callus cultures. The correlation analysis revealed that TFC was highly correlated with TPC with the maximum correlation coefficient (> 0.99) in all the tissues. It was concluded that potato tubers possessed great potential for their use in production of bioactive compounds.</p></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"18 4","pages":"595 - 605"},"PeriodicalIF":2.6000,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Analytical Methods","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s12161-024-02745-z","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Potato (Solanum tubersosum L.) is the fourth largest food crop worldwide and is rich source of minerals, essential amino acids, vitamins, and other bioactive compounds. The present study was conducted to optimize extraction conditions for achieving maximum yield of TPC, TFC, and antioxidant potential from ethanolic extracts of tubers, whole plant parts, and callus cultures in Punjab Potato-101, Kufri Pukhraj, and Lady Rosetta. Callus cultures were obtained from nodal and internodal segments of potato explants. MS medium supplemented with 2,4-D (4.0 mg L⁻¹) + kinetin (0.5 mg L⁻¹) was found to be the best medium for callus induction. The results revealed that extraction using ethanol concentration of 40% at 50 °C for 45 min was the best for extraction of TPC, TFC from tubers, whole plant parts, and callus culture in all three cultivars. The same extraction conditions also yielded maximum amount of total sugars, amino acids, and vitamin C from callus cultures of all the three cultivars. Of the three cultivars, Lady Rosetta had maximum amount of TPC, TFC, and RSA from all the plant parts and callus cultures. The correlation analysis revealed that TFC was highly correlated with TPC with the maximum correlation coefficient (> 0.99) in all the tissues. It was concluded that potato tubers possessed great potential for their use in production of bioactive compounds.

查看原文本刊更多论文

Punjab -101、Kufri Pukhraj和Lady Rosetta马铃薯不同组织和离体愈伤组织中酚类物质、类黄酮和自由基清除活性的测定

马铃薯（Solanum tubersosum L.）是世界第四大粮食作物，是矿物质、必需氨基酸、维生素和其他生物活性化合物的丰富来源。本研究优化了从Punjab -101、Kufri Pukhraj和Lady Rosetta的块茎、整株和愈伤组织培养物的乙醇提取物中获得TPC、TFC最大产量和抗氧化潜力的提取条件。从马铃薯外植体的节段和节段间获得愈伤组织培养。在MS培养基中添加2,4- d (4.0 mg L−1)+ kinetin （0.5 mg L−1）是诱导愈伤组织的最佳培养基。结果表明，在乙醇浓度为40%、温度为50°C、提取时间为45 min的条件下，3个品种的块茎、整株和愈伤组织中TPC、TFC的提取效果最佳。在相同的提取条件下，3个品种的愈伤组织中总糖、氨基酸和维生素C的提取量也最大。其中，罗塞塔夫人植株各部位和愈伤组织中TPC、TFC和RSA含量最高。相关分析显示，TFC与TPC高度相关，在所有组织中相关系数最高（> 0.99）。结果表明，马铃薯块茎在生产生物活性化合物方面具有很大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.