Revolutionizing Escherichia coli detection in real samples with digital SERS aptamer sensor technology

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-28 DOI:10.1016/j.saa.2025.126314

Mengmeng Wang , Li Zheng , Fan Sun , Qingdan Ye , Pei Liang , Kun Pang , Zihong Ye , Yufeng Wang

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Abstract

Aptamer sensors based on surface-enhanced Raman scattering (SERS) technology have demonstrated great potential in the ultrasensitive and rapid detection of Escherichia coli (E. coli). Herein, this paper presents a digital SERS aptamer sensor. This sensor integrates ordered nanoscale array synthesis technology and digital analysis technology, enabling highly sensitive and rapid bacterial quantification. The ordered monolayer gold nanosphere arrays (Au NS) can form uniform and dense “hot spots” on the silicon wafer due to their uniform spherical structures and narrow gaps. Moreover, digital SERS is adopted to further optimize the signal uniformity so as to achieve precise quantification. The sensor modules are combined together through base pairing. The aptamers labeled with Raman tags are detached from the complementary DNA due to the competition of the target substance, thus realizing the detection of E. coli. The digital SERS aptamer sensor has been verified to possess excellent selectivity and reproducibility. It has a wide dynamic linear detection range from 1.0 * 10¹ to 1.0 * 10⁹ CFU/ml and a detection limit of 0.657 CFU/ml, maintaining excellent specificity even in the presence of mixed bacterial interference. The spiked recoveries in actual samples range from 98.80 % to 99.81 %. Leveraging different aptamers and digital analysis, the sensor holds promise for food safety and environmental monitoring applications.

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革命性的大肠杆菌检测在真实样品与数字SERS适体传感器技术

基于表面增强拉曼散射（SERS）技术的适体传感器在超灵敏、快速检测大肠杆菌（E. coli）方面显示出巨大的潜力。本文提出了一种数字SERS适体传感器。该传感器集成了有序纳米级阵列合成技术和数字分析技术，实现了高灵敏度和快速的细菌定量。有序单层金纳米球阵列（Au NS）由于其均匀的球形结构和狭窄的间隙，可以在硅片上形成均匀致密的“热点”。此外，采用数字SERS进一步优化信号均匀性，实现精确量化。传感器模块通过碱基配对组合在一起。用拉曼标签标记的适体由于靶物质的竞争而与互补DNA分离，从而实现对大肠杆菌的检测。数字SERS适体传感器具有良好的选择性和重复性。动态线性检测范围为1.0 * 101 ~ 1.0 * 109 CFU/ml，检出限为0.657 CFU/ml，即使在混合细菌干扰下也能保持良好的特异性。加标回收率为98.80% ~ 99.81%。利用不同的适配体和数字分析，该传感器有望用于食品安全和环境监测应用。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.